What applications has this Mouse Anti-ADAM32 Recombinant Antibody (V2-179791) been validated for?

This antibody has been validated for use in immunocytochemistry (ICC) and immunofluorescence (IF) applications. The recommended starting dilution for IF/ICC is 8-25 µg/ml, but the optimal dilution should be determined by the end-user for their specific experimental conditions.

I am not getting a signal in my immunofluorescence experiment. What are some potential troubleshooting steps?

If you are not observing a signal, consider the following: Antibody Dilution: Your target protein may be expressed at low levels. Try a range of antibody dilutions to find the optimal concentration. Antigen Retrieval: For some tissues or cell preparations, an antigen retrieval step may be necessary to unmask the epitope. Permeabilization: Ensure that your permeabilization step is sufficient to allow the antibody to access the intracellular target. Positive Control: Use a cell line or tissue known to express ADAM32 as a positive control to confirm that your protocol and reagents are working correctly. Secondary Antibody: Verify that your secondary antibody is appropriate for detecting a mouse IgG1 primary antibody and that it is not expired.

Mouse Anti-ADAM32 Recombinant Antibody (V2-179791) (CBMAB-A1153-YC)

See all ADAM32 antibodies

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Datasheet

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Basic Information

Host Animal

Mouse

Clone

V2-179791

Application

ICC, IF

Immunogen

CHO-derived recombinant human ADAM32 Ser17-Thr476

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF(ICC)	8-25 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, Trehalose

Preservative

None

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ADAM Metallopeptidase Domain 32

Introduction

ADAM32 is a member of the disintegrin family of membrane-anchored proteins that play a role in diverse biological processes such as brain development, fertilization, tumor development and inflammation. This gene is predominantly expressed in the testis. T

Entrez Gene ID

203102

UniProt ID

Q8TC27

Alternative Names

ADAM Metallopeptidase Domain 32; A Disintegrin And Metalloproteinase Domain 32; ADAM 32;

Function

May play a role in sperm development and fertilization This is a non-catalytic metalloprotease-like protein.

Biological Process

Binding of sperm to zona pellucida
Cell adhesion

Cellular Location

Membrane

Topology

Extracellular: 175-682 aa
Helical: 683-703 aa
Cytoplasmic: 704-787 aa

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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