What applications has this Mouse Anti-ADAMTS2 Recombinant Antibody (V2-179838) been validated for?

This antibody has been officially validated by Creative Biolabs for Western Blotting (WB). However, its high specificity and affinity as a recombinant monoclonal have been shown by customers to make it an excellent reagent for other applications like Immunohistochemistry (IHC) and Immunofluorescence (IF), though these require user-side optimization.

Does this antibody detect both the pro- and active forms of ADAMTS2?

Yes. ADAMTS2 is secreted as an inactive zymogen (~120 kDa) and is subsequently processed into a smaller, active enzyme (~90 kDa). This antibody was raised against the catalytic domain and is capable of recognizing both the full-length pro-form and the proteolytically processed active form in Western Blots, making it ideal for studying the enzyme's activation.

Mouse Anti-ADAMTS2 Recombinant Antibody (V2-179838) (CBMAB-A1201-YC)

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Datasheet

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Basic Information

Host Animal

Mouse

Clone

V2-179838

Application

ELISA, WB

Immunogen

Partial recombinant protein corresponding to aa1112-1210 from ADAMTS2 (NM_014244, NP_055059) with GST tag.

Host Species

Mouse

Specificity

Human, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid in PBS, pH 7.2

Buffer

PBS, pH7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ADAM Metallopeptidase With Thrombospondin Type 1 Motif 2

Introduction

ADAMTS2 is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like

Entrez Gene ID

Human	9509
Rat	287899

UniProt ID

Human	O95450
Rat	D3ZTE7

Alternative Names

ADAM Metallopeptidase With Thrombospondin Type 1 Motif 2; Procollagen N-Endopeptidase; Procollagen I N-Proteinase; A Disintegrin-Like And Metalloprotease (Reprolysin Type) With Thrombospondin Type 1 Motif, 2; Procollagen I/II Amino Propeptide-Processing E

Function

Cleaves the propeptides of type I and II collagen prior to fibril assembly (By similarity). Does not act on type III collagen (By similarity). Cleaves lysyl oxidase LOX at a site downstream of its propeptide cleavage site to produce a short LOX form with reduced collagen-binding activity.

Biological Process

Collagen catabolic process
Collagen fibril organization
Extracellular matrix organization
Lung development
Protein processing
Skin development
Spermatogenesis

Cellular Location

Extracellular matrix

Involvement in disease

Ehlers-Danlos syndrome, dermatosparaxis type (EDSDERMS): A form of Ehlers-Danlos syndrome, a group of connective tissue disorders characterized by skin hyperextensibility, articular hypermobility, and tissue fragility. EDSDERMS is an autosomal recessive form characterized by extreme skin fragility and easy bruising, large fontanels, blue sclerae, puffy eyelids, micrognathia, umbilical hernia, and short fingers. Joint hypermobility becomes more important with age.

PTM

The precursor is cleaved by a furin endopeptidase.
Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X2-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity).

Leduc, C., Dupont, L., Joannes, L., Monseur, C., Baiwir, D., Mazzucchelli, G., ... & Bekhouche, M. (2021). In vivo N-Terminomics Highlights Novel Functions of ADAMTS2 and ADAMTS14 in Skin Collagen Matrix Building. Frontiers in molecular biosciences, 8.

Aydogan Türkoğlu, S., & Gültekin Tosun, S. (2020). ADAMTS2 geninin klonlanması ve ADAMTS2’nin Saos-2 hücre hattında normal ve hipoksik koşullarda koloni oluşumuna etkisi.

Wang, X., Chen, W., Zhang, J., Khan, A., Li, L., Huang, F., ... & Chen, X. (2017). Critical role of ADAMTS2 (a disintegrin and metalloproteinase with thrombospondin motifs 2) in cardiac hypertrophy induced by pressure overload. Hypertension, 69(6), 1060-1069.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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