Why is a recombinant antibody a better choice for my research on ADAMTSL4?

A recombinant antibody offers several key advantages over traditional monoclonals. First, superior reproducibility: it is produced from a defined DNA sequence, eliminating the batch-to-batch variability common with hybridoma production. This is critical for long-term studies or when comparing data generated at different times. Second, a guaranteed long-term supply: the antibody can be produced indefinitely. Third, high purity and specificity: the in vitro production process results in a highly pure antibody, reducing non-specific binding and background noise in your experiments.

What are the validated applications for this anti-ADAMTSL4 antibody?

This antibody has been rigorously validated by Creative Biolabs for use in Western Blotting (WB) and ELISA. While customers have reported success in other applications like Immunoprecipitation, these uses have not been formally validated in-house. For any new application, we strongly recommend performing an initial optimization and validation experiment in your own lab.

Mouse Anti-ADAMTSL4 Recombinant Antibody (V2-179860) (CBMAB-A1230-YC)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-179860

Application

ELISA, WB

Immunogen

ADAMTSL4 (AAH27478. 2, 1-424 aa) full-length recombinant protein with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, pH 7.4

Buffer

PBS, pH7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ADAMTS-like 4

Introduction

ADAMTSL4 belongs to ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs)-like gene family and encodes a protein with seven thrombospondin type 1 repeats. The thrombospondin type 1 repeat domain is found in many proteins with diverse bio

Entrez Gene ID

54507

UniProt ID

Q6UY14

Alternative Names

ADAMTS Like 4; Thrombospondin Repeat-Containing Protein 1; ADAMTSL-4; TSRC1; Thrombospondin Repeat Containing 1; ADAMTS-Like Protein 4; ECTOL2;

Function

Positive regulation of apoptosis. May facilitate FBN1 microfibril biogenesis.

Biological Process

Apoptotic process
Epithelial cell development
Extracellular matrix organization
Positive regulation of apoptotic process

Cellular Location

Extracellular matrix. Colocalizes with FMN1 microfibrils in the eye ECM.

Involvement in disease

Ectopia lentis 2, isolated, autosomal recessive (ECTOL2): An ocular abnormality characterized by partial or complete displacement of the lens from its space resulting from defective zonule formation.
Ectopia lentis et pupillae (ECTOLP): An ocular abnormality characterized by displacement of the lenses and the pupils, associated with other ocular anomalies, but without systemic manifestations. The condition is usually bilateral, with the lenses and pupils displaced in opposite directions. Additional signs include enlarged corneal diameter, increased corneal astigmatism, increased anterior chamber depth, thinning and flattening of the iris with loss of crypts, angle malformation caused by enlarged iris processes, persistent pupillary membrane, loss of zonular fibers, tilted disk, and increased axial length. Secondary manifestations include refractive errors, glaucoma, early cataract development, and retinal detachment. Membrane formation on the posterior aspect of the iris has been observed both in histologic sections and on ultrasound biomicroscopy.

PTM

N-glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X2-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs. N- and C-glycosylations can also facilitate secretion (By similarity).

Rødahl, E., Mellgren, A. E. C., Boonstra, N. E., & Knappskog, P. M. (2020). ADAMTSL4-related eye disorders.

Singh, M., Cain, S. A., & Baldock, C. (2020). Molecular Cloning, Lentiviral Transduction, and Expression of Recombinant ADAMTSL2 and ADAMTSL4. In ADAMTS Proteases (pp. 137-155). Humana, New York, NY.

Zhao, Z., Zhang, K. N., Chai, R. C., Wang, K. Y., Huang, R. Y., Li, G. Z., ... & Jiang, T. (2019). ADAMTSL4, a secreted glycoprotein, is a novel immune-related biomarker for primary glioblastoma multiforme. Disease markers, 2019.

Safi, M., Nejad, S. K., O'Hara, M., & Shankar, S. P. (2019). Ectopia Lentis et Pupillae Caused by ADAMTSL4 Pathogenic Variants and an Algorithm for Work-up. Journal of Pediatric Ophthalmology & Strabismus, (56), e45-e48.

van Bysterveldt, K. A., Al Taie, R., Ikink, W., Oliver, V. F., & Vincent, A. L. (2017). ADAMTSL4 assessment in ectopia lentis reveals a recurrent founder mutation in Polynesians. Ophthalmic genetics, 38(6), 537-543.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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