Mouse Anti-ADGRA2 Recombinant Antibody (V2-347953) (CBMAB-T0026-YJ)

Name: Mouse Anti-ADGRA2 Recombinant Antibody (V2-347953)
SKU: CBMAB-T0026-YJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-347953

Application

IHC-F, MC

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human TEM5/GPR124, Met1-Leu699.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
FC	0.25 μg/10^6 cells

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, Trehalose

Preservative

None

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 1-699

More Infomation

Target

Full Name

adhesion G protein-coupled receptor A2

Introduction

ADGRA2 is an endothelial receptor which plays a role as a WNT7-specific coactivator of canonical Wnt signaling (By similarity). It is required for normal endothelial cell sprouting and migration in the forebrain and neural tube (By similarity). ADGRA2 has a major role in establishing the blood-brain barrier (By similarity). It binds to the glycosaminoglycans heparin, heparin sulfate, chondroitin sulfate and dermatan sulfate (PubMed:16982628).

Entrez Gene ID

25960

UniProt ID

Q96PE1

Alternative Names

Adhesion G Protein-Coupled Receptor A2; Tumor Endothelial Marker 5; G-Protein Coupled Receptor 124; GPR124; TEM5; G Protein-Coupled Receptor 124; KIAA1531

Function

Endothelial receptor which functions together with RECK to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B). Plays a key role in Wnt7-specific responses, such as endothelial cell sprouting and migration in the forebrain and neural tube, and establishment of the blood-brain barrier (By similarity). Acts as a Wnt7-specific coactivator of canonical Wnt signaling: required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex. ADGRA2-tethering function does not rely on its G-protein coupled receptor (GPCR) structure but instead on its combined capacity to interact with RECK extracellularly and recruit the Dishevelled scaffolding protein intracellularly. Binds to the glycosaminoglycans heparin, heparin sulfate, chondroitin sulfate and dermatan sulfate.

Biological Process

Cell surface receptor signaling pathway
Central nervous system development
Endothelial cell migration
G protein-coupled receptor signaling pathway
Negative regulation of vascular endothelial growth factor signaling pathway
Positive regulation of canonical Wnt signaling pathway
Positive regulation of endothelial cell migration
Regulation of angiogenesis
Regulation of chemotaxis
Regulation of establishment of blood-brain barrier
Sprouting angiogenesis
Wnt signaling pathway

Cellular Location

Cell membrane; Filopodium. Enriched at lateral cell borders and also at sites of cell-ECM (extracellular matrix) contact.

Topology

Extracellular: 34-771 aa
Helical: 772-792 aa
Cytoplasmic: 793-807 aa
Helical: 808-828 aa
Extracellular: 829-832 aa
Helical: 833-853 aa
Cytoplasmic: 854-886 aa
Helical: 887-907 aa
Extracellular: 908-924 aa
Helical: 925-945 aa
Cytoplasmic: 946-1019 aa
Helical: 1020-1040 aa
Extracellular: 1041-1047 aa
Helical: 1048-1068 aa
Cytoplasmic: 1069-1338 aa

PTM

Glycosylated.
Proteolytically cleaved into two subunits, an extracellular subunit and a seven-transmembrane subunit. Cleaved by thrombin (F2) and MMP1. Also cleaved by MMP9, with lower efficiency. Presence of the protein disulfide-isomerase P4HB at the cell surface is additionally required for shedding of the extracellular subunit, suggesting that the subunits are linked by disulfide bonds. Shedding is enhanced by the growth factor FGF2 and may promote cell survival during angiogenesis.

Mamoor, S. (2021). Differential expression of G-protein coupled receptor 124 in cancers of the breast.

Langenhan, T. (2020). Adhesion G protein–coupled receptors—candidate metabotropic mechanosensors and novel drug targets. Basic & clinical pharmacology & toxicology, 126, 5-16.

Seigfried, F. A., Dietmann, P., Kühl, M., & Kühl, S. J. (2018). Expression of the adhesion G protein-coupled receptor A2 (adgra2) during Xenopus laevis development. Gene Expression Patterns, 28, 54-61.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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