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Mouse Anti-ADGRA2 Recombinant Antibody (V2-347953) (CBMAB-T0026-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to ADGRA2 (Adhesion G Protein-Coupled Receptor A2). The antibody can be used for immunoassay techniques, such as FC, CyTOF.
See all ADGRA2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-347953
Antibody Isotype
IgG2b
Application
IHC-F, MC

Basic Information

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human TEM5/GPR124, Met1-Leu699.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
FC0.25 μg/10^6 cells

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, Trehalose
Preservative
None
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 1-699

Target

Full Name
adhesion G protein-coupled receptor A2
Introduction
ADGRA2 is an endothelial receptor which plays a role as a WNT7-specific coactivator of canonical Wnt signaling (By similarity). It is required for normal endothelial cell sprouting and migration in the forebrain and neural tube (By similarity). ADGRA2 has a major role in establishing the blood-brain barrier (By similarity). It binds to the glycosaminoglycans heparin, heparin sulfate, chondroitin sulfate and dermatan sulfate (PubMed:16982628).
Entrez Gene ID
25960
UniProt ID
Q96PE1
Alternative Names
Adhesion G Protein-Coupled Receptor A2; Tumor Endothelial Marker 5; G-Protein Coupled Receptor 124; GPR124; TEM5; G Protein-Coupled Receptor 124; KIAA1531
Function
Endothelial receptor which functions together with RECK to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B). Plays a key role in Wnt7-specific responses, such as endothelial cell sprouting and migration in the forebrain and neural tube, and establishment of the blood-brain barrier (By similarity). Acts as a Wnt7-specific coactivator of canonical Wnt signaling: required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex. ADGRA2-tethering function does not rely on its G-protein coupled receptor (GPCR) structure but instead on its combined capacity to interact with RECK extracellularly and recruit the Dishevelled scaffolding protein intracellularly. Binds to the glycosaminoglycans heparin, heparin sulfate, chondroitin sulfate and dermatan sulfate.
Biological Process
Cell surface receptor signaling pathway
Central nervous system development
Endothelial cell migration
G protein-coupled receptor signaling pathway
Negative regulation of vascular endothelial growth factor signaling pathway
Positive regulation of canonical Wnt signaling pathway
Positive regulation of endothelial cell migration
Regulation of angiogenesis
Regulation of chemotaxis
Regulation of establishment of blood-brain barrier
Sprouting angiogenesis
Wnt signaling pathway
Cellular Location
Cell membrane; Filopodium. Enriched at lateral cell borders and also at sites of cell-ECM (extracellular matrix) contact.
Topology
Extracellular: 34-771 aa
Helical: 772-792 aa
Cytoplasmic: 793-807 aa
Helical: 808-828 aa
Extracellular: 829-832 aa
Helical: 833-853 aa
Cytoplasmic: 854-886 aa
Helical: 887-907 aa
Extracellular: 908-924 aa
Helical: 925-945 aa
Cytoplasmic: 946-1019 aa
Helical: 1020-1040 aa
Extracellular: 1041-1047 aa
Helical: 1048-1068 aa
Cytoplasmic: 1069-1338 aa
PTM
Glycosylated.
Proteolytically cleaved into two subunits, an extracellular subunit and a seven-transmembrane subunit. Cleaved by thrombin (F2) and MMP1. Also cleaved by MMP9, with lower efficiency. Presence of the protein disulfide-isomerase P4HB at the cell surface is additionally required for shedding of the extracellular subunit, suggesting that the subunits are linked by disulfide bonds. Shedding is enhanced by the growth factor FGF2 and may promote cell survival during angiogenesis.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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