Does this antibody confirm the lack of autoproteolytic cleavage for ADGRF4, and what is the expected WB size?

Yes. ADGRF4 is one of the few aGPCRs thought not to undergo cleavage at its GPS site. This antibody is validated to show a single, strong band at the protein's predicted core molecular weight of approximately 77 kDa in WB (with slight variation due to glycosylation), confirming the presence of the intact receptor.

Is the antibody validated to detect ADGRF4 in its unique intracellular/cytoskeletal localization in addition to the cell membrane?

Yes. Unlike most aGPCRs, ADGRF4 is known to localize intracellularly along keratin filaments in epithelial cells. This antibody has been successfully validated in Immunofluorescence (IF) to show this unique cytoskeletal co-localization, requiring fixation and permeabilization protocols optimized for intracellular antigen access.

Mouse Anti-ADGRF4 Recombinant Antibody (V2-66357) (CBMAB-G0003-LY)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-66357

Application

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human GPR115, Ser22-Ala347

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	2 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, Trehalose

Preservative

None

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

adhesion G protein-coupled receptor F4

Introduction

Sequence analysis of this gene suggests that it is encodes a member of the superfamily of G protein-couple receptors. G protein-coupled receptors typically contain seven hydrophobic transmembrane domains, interact with guanine nucleotide binding regulatory proteins, and detect molecules outside the cell and act to transduce these signals into intracellular responses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2016]

Entrez Gene ID

221393

UniProt ID

Q8IZF3

Alternative Names

Adhesion G Protein-Coupled Receptor F4; G-Protein Coupled Receptor PGR18; GPR115; PGR18; Probable G-Protein Coupled Receptor 115; Seven Transmembrane Helix Receptor; G Protein-Coupled Receptor 115; G-Protein Coupled Receptor 115;

Function

Orphan receptor.

Biological Process

Cell surface receptor signaling pathway
G protein-coupled receptor signaling pathway

Cellular Location

Membrane

Topology

Extracellular: 22-406 aa
Helical: 407-427 aa
Cytoplasmic: 428-440 aa
Helical: 441-461 aa
Extracellular: 462-485 aa
Helical: 486-506 aa
Cytoplasmic: 507-515 aa
Helical: 516-536 aa
Extracellular: 537-561 aa
Helical: 562-582 aa
Cytoplasmic: 583-606 aa
Helical: 607-627 aa
Extracellular: 628-634 aa
Helical: 635-655 aa
Cytoplasmic: 656-695 aa

Rosa, M., Noel, T., Harris, M., & Ladds, G. (2021). Emerging roles of adhesion G protein-coupled receptors. Biochemical Society Transactions.

Chiba, Y., Yoshizaki, K., Saito, K., Ikeuchi, T., Iwamoto, T., Rhodes, C., ... & Fukumoto, S. (2020). G protein–coupled receptor Gpr115 (Adgrf4) is required for enamel mineralization mediated by ameloblasts. Journal of Biological Chemistry, 295(45), 15328-15341.

Yoon, J. H., & Cho, S. G. (2020). ADGRF4 Regulates Non-small Cell Lung Cancer Cell Invasiveness. Anticancer Research, 40(12), 6835-6844.

Langenhan, T. (2020). Adhesion G protein–coupled receptors—candidate metabotropic mechanosensors and novel drug targets. Basic & clinical pharmacology & toxicology, 126, 5-16.

Wang, Y., Shi, M., Yang, N., Zhou, X., & Xu, L. (2020). GPR115 Contributes to Lung Adenocarcinoma Metastasis Associated With LAMC2 and Predicts a Poor Prognosis. Frontiers in Oncology, 10, 2414.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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