Mouse Anti-ADGRL4 Monoclonal Antibody (V2-5386) (CBMAB-1134-YC)

Provided herein is a mouse monoclonal antibody against Human ADGRL4. The antibody, clone CL4164, can be used for immunoassay techniques, such as IHC and WB.
See all ADGRL4 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

V2-5386

Antibody Isotype

IgG1

Application

IHC, WB

Basic Information

Immunogen

Recombinant Human ELTD1

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC	1:500-1:1,000
WB	1 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2, 40% glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Purity

>95%, as determined by SDS-PAGE analysis

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Adhesion G Protein-Coupled Receptor L4

Introduction

ADGRL4 is an endothelial orphan receptor that acts as a key regulator of angiogenesis.

Entrez Gene ID

64123

UniProt ID

Q9HBW9

Alternative Names

ETL; ELTD1; KPG_003

Function

Endothelial orphan receptor that acts as a key regulator of angiogenesis.

Biological Process

Adenylate cyclase-activating G protein-coupled receptor signaling pathway
Cell surface receptor signaling pathway
G protein-coupled receptor signaling pathway
Protein-containing complex assembly

Cellular Location

Cell membrane

Topology

Extracellular: 20-432 aa
Helical: 433-453 aa
Cytoplasmic: 454-460 aa
Helical: 461-481 aa
Extracellular: 482-499 aa
Helical: 500-520 aa
Cytoplasmic: 521-532 aa
Helical: 533-553 aa
Extracellular: 554-573 aa
Helical: 574-594 aa
Cytoplasmic: 595-618 aa
Helical: 619-639 aa
Extracellular: 640-646 aa
Helical: 647-667 aa
Cytoplasmic: 668-690 aa

PTM

Glycosylated.
Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.

References

Towner, R. A., Zalles, M., Saunders, D., & Smith, N. (2020). Novel approaches to combat chemoresistance against glioblastomas. Cancer Drug Resistance, 3(4), 686-698.

Langenhan, T. (2020). Adhesion G protein–coupled receptors—candidate metabotropic mechanosensors and novel drug targets. Basic & clinical pharmacology & toxicology, 126, 5-16.

Favara, D. M., Zois, C. E., Haider, S., Pires, E., Sheldon, H., McCullagh, J., ... & Harris, A. L. (2019). ADGRL4/ELTD1 silencing in endothelial cells induces ACLY and SLC25A1 and alters the cellular metabolic profile. Metabolites, 9(12), 287.

Favara, D. M. (2017). The biology of ELTD1/ADGRL4: a novel regulator of tumour angiogenesis (Doctoral dissertation, University of Oxford).

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Datasheet
SDS
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Second antibody and isotype control

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