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Rabbit Anti-ADHFE1 Recombinant Antibody (V2-179915) (CBMAB-A1299-YC)

Provided herein is a Rabbit monoclonal antibody against Human Alcohol Dehydrogenase, Iron Containing 1. The antibody can be used for immunoassay techniques, such as WB, IP.
See all ADHFE1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human
Clone
V2-179915
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Immunogen
Recombinant fragment within Human ADHFE1.
Host Species
Rabbit
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000-1:10,000
IP1:10-1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Buffer
9% PBS, 40% Glycerol, 0.05% BSA
Preservative
0.01% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Alcohol Dehydrogenase, Iron Containing 1
Introduction
ADHFE1 is hydroxyacid-oxoacid transhydrogenase (EC 1.1.99.24), which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues.
Entrez Gene ID
UniProt ID
Alternative Names
Alcohol Dehydrogenase, Iron Containing 1; Alcohol Dehydrogenase Iron-Containing Protein 1; EC 1.1.99.24; HOT; Hydroxyacid-Oxoacid Transhydrogenase, Mitochondrial; Fe-Containing Alcohol Dehydrogenase 1; Hydroxyacid-Oxoacid Transhydrogenase;
Function
Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). D,L-3-hydroxyisobutyrate and L-3-hydroxybutyrate (L-3-OHB) are also substrates for HOT with 10-fold lower activities.
Biological Process
2-oxoglutarate metabolic process
Glutamate catabolic process via 2-oxoglutarate
Cellular Location
Mitochondrion
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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