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Mouse Anti-ADO Recombinant Antibody (V2-180004) (CBMAB-A1391-YC)

Provided herein is a Mouse monoclonal antibody against Human 2-Aminoethanethiol Dioxygenase. The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all ADO antibodies
Published Data
Mouse Anti-ADO Recombinant Antibody (E-11) (CBMAB-A1391-YC) in Immunoblot
ADO knockout cells were generated by utilizing CRISPR-Cas9 system in LN229. ...View More
Shen, D., Tian, L., Yang, F., Li, J., Li, X., Yao, Y., ... & Wang, R. (2021). ADO/hypotaurine: A novel metabolic pathway contributing to glioblastoma development. Cell Death Discovery, 7(1), 21.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
V2-180004
Antibody Isotype
IgG2b, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Specific for an epitope mapping between amino acids 240-258 near the C-terminus of ADO of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate).
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, 0.1% gelatin.
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
2-Aminoethanethiol Dioxygenase
Introduction
Human thiol dioxygenases include cysteine dioxygenase and cysteamine (2-aminoethanethiol) dioxygenase. CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine.
Entrez Gene ID
Human84890
Mouse211488
Rat309732
UniProt ID
HumanQ96SZ5
Alternative Names
2-Aminoethanethiol Dioxygenase; Cysteamine Dioxygenase; 2-Aminoethanethiol (Cysteamine) Dioxygenase; C10orf22; Cysteamine (2-Aminoethanethiol) Dioxygenase (ADO); Chromosome 10 Open Reading Frame 22; EC 1.13.11.19;
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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