Sign in or Register   Sign in or Register
  |  

Mouse Anti-ADPGK Recombinant Antibody (V2-180015) (CBMAB-A1402-YC)

Provided herein is a Mouse monoclonal antibody against Human ADP Dependent Glucokinase. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all ADPGK antibodies
Published Data
Fig. 1 Western blots for validation of a monoclonal antibody for ADPGK, demonstrating recognition of its C-terminal 72 amino acids expressed as a GST fusion (rhADPGK[425–496]GST). The antibody detects a ~54 kDa band in human tumour cell lines H460 and HCT116 and in three clones from each line overexpressing full-length hADPGK.
...View More

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
V2-180015
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
Partial recombinant corresponding to aa 425-497 from human ADPGK (NP_112574) with GST tag.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid in PBS, pH 7.2
Buffer
PBS, pH7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ADP Dependent Glucokinase
Introduction
ADPGK (EC 2.7.1.147) catalyzes the ADP-dependent phosphorylation of glucose to glucose-6-phosphate and may play a role in glycolysis, possibly during ischemic conditions.
Entrez Gene ID
Human83440
Mouse72141
Rat315722
UniProt ID
HumanQ9BRR6
MouseQ8VDL4
RatG3V784
Alternative Names
ADP Dependent Glucokinase; RbBP-35; ADP-GK; ADP-Dependent Glucokinase; ATP-Dependent Glucokinase; 2610017G09Rik; EC 2.7.1.147;
Function
Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency (By similarity).
Biological Process
Glucose metabolic process
Glycolytic process through glucose-6-phosphate
Cellular Location
Secreted

Mamoor, S. (2021). ADPGK is differentially expressed in brain metastatic human breast cancer.

Tandon, A., Birkenhagen, J., Nagalla, D., Kölker, S., & Sauer, S. W. (2020). ADP-dependent glucokinase as a novel onco-target for haematological malignancies. Scientific reports, 10(1), 1-16.

Imle, R. (2019). ADP-dependent Glucokinase–a novel regulator of ER-mediated glucose handling.

Imle, R., Wang, B. T., Stützenberger, N., Birkenhagen, J., Tandon, A., Carl, M., ... & Sauer, S. W. (2019). ADP-dependent glucokinase regulates energy metabolism via ER-localized glucose sensing. Scientific reports, 9(1), 1-12.

Yang, J., Wu, W., Wu, M., & Ding, J. (2019). Long noncoding RNA ADPGK-AS1 promotes cell proliferation, migration, and EMT process through regulating miR-3196/OTX1 axis in breast cancer. In Vitro Cellular & Developmental Biology-Animal, 55(7), 522-532.

Tokarz, P., Wiśniewska, M., Kamiński, M. M., Dubin, G., & Grudnik, P. (2018). Crystal structure of ADP‐dependent glucokinase from Methanocaldococcus jannaschii in complex with 5‐iodotubercidin reveals phosphoryl transfer mechanism. Protein Science, 27(3), 790-797.

Grudnik, P., Kamiński, M. M., Rembacz, K. P., Kuśka, K., Madej, M., Potempa, J., ... & Dubin, G. (2018). Structural basis for ADP-dependent glucokinase inhibition by 8-bromo–substituted adenosine nucleotide. Journal of Biological Chemistry, 293(28), 11088-11099.

Song, S., Yu, W., Lin, S., Zhang, M., Wang, T., Guo, S., & Wang, H. (2018). LncRNA ADPGK-AS1 promotes pancreatic cancer progression through activating ZEB1-mediated epithelial–mesenchymal transition. Cancer biology & therapy, 19(7), 573-583.

Rey, S., Schito, L., Koritzinsky, M., & Wouters, B. G. (2017). ADP-dependent glucokinase controls hypoxic gradients, ex vivo avascular and in vivo tumor growth through modulation of HIF-1α/mTOR signaling.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-ADPGK Recombinant Antibody (V2-180015)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare