Mouse Anti-ADV-5 E1A Recombinant Antibody (V2-503467) (CBMAB-V208-1382-FY)

Name: Mouse Anti-ADV-5 E1A Recombinant Antibody (V2-503467)
SKU: CBMAB-V208-1382-FY
Rating: 5 (1 reviews)

See all ADV-5 E1A antibodies

Compare Online Inquiry

Published Data

Mouse Anti-ADV-5 E1A Recombinant Antibody (CBMW-H1211) (CBMAB-V208-1382-FY) in WB

Western blot analyses of E1AΔpRb, TRAIL, and Mcl-1 in the melanoma cell lines MeWo and Mel-HO. The cells were transfected with 30 nM of a control siRNA (siCon) or siMcl-1 and infected 48 h later with AdV-TRAIL at a MOI of 5 (MeWo) and 2 (Mel-HO). Simultaneously, TRAIL expression was induced by Dox as indicated. Proteins were extracted 24 h after infection and expression of Mcl-1, TRAIL, and E1AΔpRb was determined. As an internal loading control, β-actin was used. Representative data from three independent experiments are shown. ...View More

Tolksdorf, B., Zarif, S., Eberle, J., Hazini, A., Dieringer, B., Jönsson, F., ... & Fechner, H. (2021). Silencing of Mcl-1 overcomes resistance of melanoma cells against TRAIL-armed oncolytic adenovirus by enhancement of apoptosis. Journal of Molecular Medicine, 99(9), 1279-1291.

Mouse Anti-ADV-5 E1A Recombinant Antibody (CBMW-H1211) (CBMAB-V208-1382-FY) in Immunoblot

Cells were either left uninfected (Ctrl) or infected with AdGFP or OAdmCherry at a multiplicity of infection (MOI) concentration of five alone or in combination with 500 nM palbociclib for 24 h. Cyclin E and viral E1A expression was assessed by immunoblotting. ...View More

Lypova, N., Lanceta, L., Gipson, A., Vega, S., Garza-Morales, R., McMasters, K. M., ... & Imbert-Fernandez, Y. (2019). Targeting palbociclib-resistant estrogen receptor-positive breast cancer cells via oncolytic virotherapy. Cancers, 11(5), 684.

Mouse Anti-ADV-5 E1A Recombinant Antibody (CBMW-H1211) (CBMAB-V208-1382-FY) in IP

Specific immunoprecipitation of endogenous p300. (Left panel) Interaction of endogenous (end) p300 with transfected E1A. FaDu cells were transfected with Flag-E1A and Flag-E1B expression vectors, the lysates (lys) were subjected to immunoprecipitation (IP) with an anti-Flag antibody. Proteins detected in western blotting, as well as the positions of size markers (in kilodaltons) are indicated. SUMO-ΔNp63α is also marked. (Right panel) Detection of endogenous p300-p63 complex formation. FaDu cells were transfected with Flag-E1A, followed by lysis and immunoprecipitation with an anti-p300 antibody. ...View More

Katoh, I., Maehata, Y., Moriishi, K., Hata, R. I., & Kurata, S. I. (2019). C-terminal α domain of p63 binds to p300 to coactivate β-catenin. Neoplasia, 21(5), 494-503.

Mouse Anti-ADV-5 E1A Recombinant Antibody (CBMW-H1211) (CBMAB-V208-1382-FY) in IF

E1A protein (green) was visualized using immunofluorescence in PHH 345 at day 4 and day 7 p.p. Scale bar represents 50 μm. ...View More

Moreau, P., Cournac, A., Palumbo, G. A., Marbouty, M., Mortaza, S., Thierry, A., ... & Neuveut, C. (2018). Tridimensional infiltration of DNA viruses into the host genome shows preferential contact with active chromatin. Nature communications, 9(1), 4268.

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-503467

Application

IF, IHC, IP, WB

Immunogen

Adenovirus-5 E1A

Host Species

Mouse

Specificity

ADV

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate).
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Adenovirus Type 5 E1A

Introduction

Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E1A protein has both transforming and trans-activating activities.

UniProt ID

P03255

Alternative Names

Hadv 5 E1A; HAdv5 E1A

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-ADV-5 E1A Recombinant Antibody (V2-503467)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry