What is the primary function and localization of the AIDA protein?

AIDA (Axin Interactor, Dorsalization Associated) is a scaffolding protein primarily known for its role in the Wnt/JNK signaling pathway. Its main function is to inhibit Axin-mediated JNK activation. It does this by binding directly to Axin and disrupting its homodimerization, which is required for JNK signaling. AIDA is localized primarily in the cytoplasm, which is where it interacts with the Axin protein complex.

What is the expected molecular weight of AIDA in a Western Blot?

The canonical human AIDA protein (isoform 1) has a calculated molecular weight of approximately ~35 kDa. You should look for a single, specific band in this region. Other isoforms have been reported but are less common, so the ~35 kDa band is the expected target.

Mouse Anti-AIDA Recombinant Antibody (V2-607132) (CBMAB-C9464-LY)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

V2-607132

Application

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Concentration

1 mg/mL

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Axin Interactor, Dorsalization Associated

Introduction

Acts as a ventralizing factor during embryogenesis. Inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling (By similarity).

Entrez Gene ID

64853

UniProt ID

Q96BJ3

Alternative Names

Axin Interactor, Dorsalization Associated; Axin Interaction Partner And Dorsalization Antagonist; C1orf80; Axin Interactor, Dorsalization-Associated Protein; Chromosome 1 Open Reading Frame 80;

Function

Biological Process

Determination of ventral identity Source: GO_Central
Dorsal/ventral pattern formation Source: UniProtKB
Negative regulation of JNK cascade Source: UniProtKB
Negative regulation of JUN kinase activity Source: MGI
Negative regulation of protein-containing complex assembly Source: UniProtKB

Cellular Location

Cytoplasm; Membrane

Lalonde, S., Codina-Fauteux, V. A., de Bellefon, S. M., Leblanc, F., Beaudoin, M., Simon, M. M., ... & Lettre, G. (2019). Integrative analysis of vascular endothelial cell genomic features identifies AIDA as a coronary artery disease candidate gene. Genome biology, 20(1), 1-13.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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