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Mouse Anti-AK3 Recombinant Antibody (V2-180400) (CBMAB-A1872-YC)

Provided herein is a Mouse monoclonal antibody against Human Adenylate Kinase 3. The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all AK3 antibodies
Published Data
Mouse Anti-AK3 Recombinant Antibody (E-5) in WB
Western blot analysis revealed the increased expression of AK4 protein in TamR MCF-7 cells. ...View More
Liu, X., Gonzalez, G., Dai, X., Miao, W., Yuan, J., Huang, M., ... & Wang, Y. (2020). Adenylate kinase 4 modulates the resistance of breast cancer cells to tamoxifen through an m6A-based epitranscriptomic mechanism. Molecular Therapy, 28(12), 2593-2604.
Mouse Anti-AK3 Recombinant Antibody (E-5) in IF
Validation of candidates by immunofluorescence staining in cerebellum. Candidates show prominent colocalization with cell-type-specific GFP-OMM (green), confirming the proteomics data astrocytes. ...View More
Fecher, C., Trovò, L., Müller, S. A., Snaidero, N., Wettmarshausen, J., Heink, S., ... & Misgeld, T. (2019). Cell-type-specific profiling of brain mitochondria reveals functional and molecular diversity. Nature neuroscience, 22(10), 1731-1742.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
V2-180400
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 209-227 at the C-terminus of AK3 of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
adenylate kinase 3
Introduction
AK3 is a GTP:ATP phosphotransferase that is found in the mitochondrial matrix. Several transcript variants encoding a few different isoforms have been found for this gene.
Entrez Gene ID
Human50808
Mouse56248
Rat26956
UniProt ID
HumanQ9UIJ7
MouseQ9WTP7
RatP29411
Alternative Names
Adenylate Kinase 3; Adenylate Kinase 3 Alpha-Like 1; EC 2.7.4.10; AK3L1; AKL3L; AK6; GTP:AMP Phosphotransferase AK3, Mitochondrial; Adenylate Kinase 6, Adenylate Kinase 3 Like 1;
Function
Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities.
Biological Process
ADP biosynthetic process Source: UniProtKB-UniRule
AMP metabolic process Source: BHF-UCL
Blood coagulation Source: Reactome
GTP metabolic process Source: BHF-UCL
ITP metabolic process Source: BHF-UCL
Nucleoside monophosphate phosphorylation Source: GOC
Nucleoside triphosphate biosynthetic process Source: GO_Central
UTP metabolic process Source: BHF-UCL
Cellular Location
Mitochondrion matrix
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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