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Mouse Anti-ALOX12 Recombinant Antibody (1B2) (CBMAB-A2367-YC)

Provided herein is a Mouse monoclonal antibody against Human Arachidonate 12-Lipoxygenase, 12S Type. The antibody can be used for immunoassay techniques, such as IHC.
See all ALOX12 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1B2
Antibody Isotype
IgG1
Application
IHC, WB, IF, ICC

Basic Information

Immunogen
Full length human recombinant protein of human ALOX12 (NP_000688) produced in HEK293T cell
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB0.2-2 µg/ml
IHC5-20 µg/ml
IF(ICC)5-20 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH7.4, 50% glycerol
Preservative
0.02% sodium azide
Concentration
1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Arachidonate 12-Lipoxygenase, 12S Type
Introduction
ALOX12 is a member of the lipoxygenase family of proteins. The encoded enzyme acts on different polyunsaturated fatty acid substrates to generate bioactive lipid mediators including eicosanoids and lipoxins. The encoded enzyme and its reaction products ha
Entrez Gene ID
239
UniProt ID
P18054
Alternative Names
Arachidonate 12-Lipoxygenase, 12S Type; Arachidonate 12-Lipoxygenase, 12S-Type; Platelet-Type Lipoxygenase 12; Lipoxin Synthase 12-LO; 12S-Lipoxygenase; Platelet 12-LOX; 12S-LOX; LOG12;
Function
Catalyzes the regio and stereo-specific incorporation of molecular oxygen into free and esterified polyunsaturated fatty acids generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species (PubMed:17493578, PubMed:1851637, PubMed:8319693, PubMed:8500694, PubMed:18311922, PubMed:32404334). Mainly converts arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) to the specific bioactive lipid (12S)-hydroperoxyeicosatetraenoate/(12S)-HPETE (PubMed:17493578, PubMed:22984144, PubMed:24282679, PubMed:8319693, PubMed:8500694). Through the production of bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation (PubMed:8319693, PubMed:8500694). It can also catalyze the epoxidation of double bonds of polyunsaturated fatty acids such as (14S)-hydroperoxy-docosahexaenoate/(14S)-HPDHA resulting in the formation of (13S,14S)-epoxy-DHA (PubMed:23504711). Furthermore, it may participate in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs) like resolvin D5 ((7S,17S)-diHPDHA) and (7S,14S)-diHPDHA, that actively downregulate the immune response and have anti-aggregation properties with platelets (PubMed:32404334). An additional function involves a multistep process by which it transforms leukotriene A4/LTA4 into the bioactive lipids lipoxin A4/LXA4 and lipoxin B4/LXB4, both are vasoactive and LXA4 may regulate neutrophil function via occupancy of specific recognition sites (PubMed:8250832). Can also peroxidize linoleate ((9Z,12Z)-octadecadienoate) to (13S)-hydroperoxyoctadecadienoate/ (13S-HPODE) (By similarity). Due to its role in regulating both the expression of the vascular endothelial growth factor (VEGF, an angiogenic factor involved in the survival and metastasis of solid tumors) and the expression of integrin beta-1 (known to affect tumor cell migration and proliferation), it can be regarded as protumorigenic (PubMed:9751607, PubMed:16638750, PubMed:22237009). Important for cell survival, as it may play a role not only in proliferation but also in the prevention of apoptosis in vascular smooth muscle cells (PubMed:23578768).
Biological Process
Aging Source: Ensembl
Arachidonic acid metabolic process Source: UniProtKB
Cellular response to lipid Source: Ensembl
Establishment of skin barrier Source: UniProtKB
Fatty acid oxidation Source: UniProtKB
Hepoxilin biosynthetic process Source: UniProtKB
Hepoxilin metabolic process Source: Reactome
Leukotriene A4 metabolic process Source: UniProtKB
Linoleic acid metabolic process Source: UniProtKB
Lipid metabolic process Source: UniProtKB
Lipid oxidation Source: GO_Central
Lipoxin A4 biosynthetic process Source: UniProtKB
Lipoxin B4 biosynthetic process Source: UniProtKB
Lipoxin biosynthetic process Source: Reactome
Lipoxygenase pathway Source: UniProtKB
Long-chain fatty acid biosynthetic process Source: Reactome
Negative regulation of muscle cell apoptotic process Source: UniProtKB
Negative regulation of platelet aggregation Source: UniProtKB
Positive regulation of apoptotic process Source: Ensembl
Positive regulation of blood vessel endothelial cell migration Source: Ensembl
Positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
Positive regulation of endothelial tube morphogenesis Source: Ensembl
Positive regulation of gene expression Source: Ensembl
Positive regulation of mitochondrial depolarization Source: Ensembl
Positive regulation of smooth muscle cell proliferation Source: Ensembl
Superoxide anion generation Source: UniProtKB
Unsaturated fatty acid metabolic process Source: UniProtKB
Cellular Location
Cytosol; Membrane. Membrane association is stimulated by EGF.
Involvement in disease
Esophageal cancer (ESCR): Disease susceptibility may be associated with variants affecting the gene represented in this entry. Gln at position 261 may confer interindividual susceptibility to esophageal cancer (PubMed:17460548). A malignancy of the esophagus. The most common types are esophageal squamous cell carcinoma and adenocarcinoma. Cancer of the esophagus remains a devastating disease because it is usually not detected until it has progressed to an advanced incurable stage.
Colorectal cancer (CRC): Disease susceptibility may be associated with variants affecting the gene represented in this entry. Gln at position 261 may confer interindividual susceptibility to colorectal cancer (PubMed:17460548). A complex disease characterized by malignant lesions arising from the inner wall of the large intestine (the colon) and the rectum. Genetic alterations are often associated with progression from premalignant lesion (adenoma) to invasive adenocarcinoma. Risk factors for cancer of the colon and rectum include colon polyps, long-standing ulcerative colitis, and genetic family history.

Sabbir, M. G., Taylor, C. G., & Zahradka, P. (2021). Antisense overlapping long non-coding RNA regulates coding arachidonate 12-lipoxygenase gene by translational interference. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 1866(10), 158987.

Mitsui, T., Makino, S., Tamiya, G., Sato, H., Kawakami, Y., Takahashi, Y., ... & Hayasaka, K. (2021). ALOX12 mutation in a family with dominantly inherited bleeding diathesis. Journal of Human Genetics, 1-7.

Mamoor, S. (2020). Arachidonate 12-lipoxygenase (ALOX12) is differentially expressed in the tumors of breast cancer patients treated with trastuzumab.

Zheng, Z., Li, Y., Jin, G., Huang, T., Zou, M., & Duan, S. (2020). The biological role of arachidonic acid 12-lipoxygenase (ALOX12) in various human diseases. Biomedicine & Pharmacotherapy, 129, 110354.

Chung, E. J., Reedy, J. L., Kwon, S., Patil, S., Valle, L., White, A. O., & Citrin, D. E. (2019). 12-lipoxygenase is a critical mediator of type II pneumocyte senescence, macrophage polarization and pulmonary fibrosis after irradiation. Radiation research, 192(4), 367-379.

Huang, Z., Xia, L., Zhou, X., Wei, C., & Mo, Q. (2019). ALOX12 inhibition sensitizes breast cancer to chemotherapy via AMPK activation and inhibition of lipid synthesis. Biochemical and biophysical research communications, 514(1), 24-30.

Kizawa, K., Fujimori, T., & Kawai, T. (2017). Arachidonate 12-lipoxygenase inhibitors promote S100A3 citrullination in cultured SW480 cells and isolated hair follicles. Biological and Pharmaceutical Bulletin, 40(4), 516-523.

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For research use only. Not intended for any clinical use.

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