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Mouse Anti-ALOX12B Recombinant Antibody (3G12) (CBMAB-A2371-YC)

Provided herein is a Mouse monoclonal antibody against Human Arachidonate 12-Lipoxygenase, 12R Type. The antibody can be used for immunoassay techniques, such as ELISA.
See all ALOX12B antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3G12
Antibody Isotype
IgG2a, κ
Application
ELISA

Basic Information

Immunogen
Partial recombinant corresponding to aa 171-261 from human ALOX12B (NP_001130) with GST tag.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
arachidonate 12-lipoxygenase, 12R type
Introduction
ALOX12B is an enzyme involved in the conversion of arachidonic acid to 12R-hydroxyeicosatetraenoic acid. Mutations in this gene are associated with nonbullous congenital ichthyosiform erythroderma.
Entrez Gene ID
242
UniProt ID
O75342
Alternative Names
Arachidonate 12-Lipoxygenase, 12R Type; Epidermis-Type Lipoxygenase 12; 12R-Lipoxygenase; 12R-LOX; Arachidonate 12-Lipoxygenase, 12R-Type; EC 1.13.11.33; EC 1.13.11.-; ARCI2;
Function
Catalyzes the regio and stereo-specific incorporation of a single molecule of dioxygen into free and esterified polyunsaturated fatty acids generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species (PubMed:9837935, PubMed:9618483, PubMed:21558561). In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins (PubMed:21558561). Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss (PubMed:21558561). May also play a role in the regulation of the expression of airway mucins (PubMed:22441738).
Biological Process
Arachidonic acid metabolic process Source: UniProtKB
Ceramide biosynthetic process Source: UniProtKB
Establishment of skin barrier Source: UniProtKB
Hepoxilin biosynthetic process Source: UniProtKB
Linoleic acid metabolic process Source: UniProtKB
Lipid oxidation Source: GO_Central
Lipoxygenase pathway Source: UniProtKB
Positive regulation of gene expression Source: UniProtKB
Positive regulation of MAPK cascade Source: UniProtKB
Positive regulation of mucus secretion Source: UniProtKB
Protein lipidation Source: UniProtKB
Sphingolipid metabolic process Source: UniProtKB
Cellular Location
Cytoplasm; Perinuclear region
Involvement in disease
Ichthyosis, congenital, autosomal recessive 2 (ARCI2): A form of autosomal recessive congenital ichthyosis, a disorder of keratinization with abnormal differentiation and desquamation of the epidermis, resulting in abnormal skin scaling over the whole body. The main skin phenotypes are lamellar ichthyosis (LI) and non-bullous congenital ichthyosiform erythroderma (NCIE), although phenotypic overlap within the same patient or among patients from the same family can occur. Lamellar ichthyosis is a condition often associated with an embedment in a collodion-like membrane at birth; skin scales later develop, covering the entire body surface. Non-bullous congenital ichthyosiform erythroderma characterized by fine whitish scaling on an erythrodermal background; larger brownish scales are present on the buttocks, neck and legs.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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