Mouse Anti-ALOX12B Recombinant Antibody (3G12) (CBMAB-A2371-YC)

Name: Mouse Anti-ALOX12B Recombinant Antibody (3G12)
SKU: CBMAB-A2371-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3G12

Application

ELISA

Immunogen

Partial recombinant corresponding to aa 171-261 from human ALOX12B (NP_001130) with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

arachidonate 12-lipoxygenase, 12R type

Introduction

ALOX12B is an enzyme involved in the conversion of arachidonic acid to 12R-hydroxyeicosatetraenoic acid. Mutations in this gene are associated with nonbullous congenital ichthyosiform erythroderma.

Entrez Gene ID

242

UniProt ID

O75342

Alternative Names

Arachidonate 12-Lipoxygenase, 12R Type; Epidermis-Type Lipoxygenase 12; 12R-Lipoxygenase; 12R-LOX; Arachidonate 12-Lipoxygenase, 12R-Type; EC 1.13.11.33; EC 1.13.11.-; ARCI2;

Function

Catalyzes the regio and stereo-specific incorporation of a single molecule of dioxygen into free and esterified polyunsaturated fatty acids generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species (PubMed:9837935, PubMed:9618483, PubMed:21558561). In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins (PubMed:21558561). Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss (PubMed:21558561). May also play a role in the regulation of the expression of airway mucins (PubMed:22441738).

Biological Process

Arachidonic acid metabolic process Source: UniProtKB
Ceramide biosynthetic process Source: UniProtKB
Establishment of skin barrier Source: UniProtKB
Hepoxilin biosynthetic process Source: UniProtKB
Linoleic acid metabolic process Source: UniProtKB
Lipid oxidation Source: GO_Central
Lipoxygenase pathway Source: UniProtKB
Positive regulation of gene expression Source: UniProtKB
Positive regulation of MAPK cascade Source: UniProtKB
Positive regulation of mucus secretion Source: UniProtKB
Protein lipidation Source: UniProtKB
Sphingolipid metabolic process Source: UniProtKB

Cellular Location

Cytoplasm; Perinuclear region

Involvement in disease

Ichthyosis, congenital, autosomal recessive 2 (ARCI2): A form of autosomal recessive congenital ichthyosis, a disorder of keratinization with abnormal differentiation and desquamation of the epidermis, resulting in abnormal skin scaling over the whole body. The main skin phenotypes are lamellar ichthyosis (LI) and non-bullous congenital ichthyosiform erythroderma (NCIE), although phenotypic overlap within the same patient or among patients from the same family can occur. Lamellar ichthyosis is a condition often associated with an embedment in a collodion-like membrane at birth; skin scales later develop, covering the entire body surface. Non-bullous congenital ichthyosiform erythroderma characterized by fine whitish scaling on an erythrodermal background; larger brownish scales are present on the buttocks, neck and legs.

Hotz, A., Kopp, J., Bourrat, E., Oji, V., Komlosi, K., Giehl, K., ... & Fischer, J. (2021). Meta-Analysis of Mutations in ALOX12B or ALOXE3 Identified in a Large Cohort of 224 Patients. Genes, 12(1), 80.

Dawman, L., Kaur, A., Nada, R., Chakraborty, S., Handa, S., Sharawat, I. K., & Tiewsoh, K. (2020). Autosomal Recessive Congenital Ichthyosis and Steroid-Resistant Nephrotic Syndrome due to Homozygous Mutation in the ALOX12B gene: A Novel Association with Review of Literature. Journal of Pediatric Genetics.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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