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Mouse Anti-ALOX15B Recombinant Antibody (D9) (CBMAB-L0003-YC)

Provided herein is a Mouse monoclonal antibody against Human ALOX15B. The antibody can be used for immunoassay techniques, such as WB, ICC, IHC-P, IHC-Fr, ELISA.
See all ALOX15B antibodies
Published Data
Mouse Anti-ALOX15B Recombinant Antibody (D9) in Immunoblot
Immunoblot analysis of ALOX15B. (A): Immunoblotting of HEK293 cells transiently transfected with control expression plasmid (Empty), Flagged-tagged ALOX15 expression plasmid or Flag-tagged ALOX15B expression plasmid. Antibodies employed were anti-Flag (upper panel), anti-ALOX15 (middle panel) and anti-ALOX15B (bottom panel). Representative blots are shown. Bands are identified by their molecular mass based on the markers (M). (B): Immunoblotting of lysates prepared from confluent or non-confluent EA.hy926 cells with anti-ALOX15B antibody (upper panel). Protein loading is shown by Oriole staining (lower panel). ...View More
Sabbir, M. G., Wigle, J. T., Taylor, C. G., & Zahradka, P. (2022). Growth state-dependent expression of arachidonate lipoxygenases in the human endothelial cell line EA. hy926. Cells, 11(16), 2478.
Mouse Anti-ALOX15B Recombinant Antibody (D9) in WB
Protein expression of 5-LO, 15-LO-1, 15-LO-2 and FLAP in freshly isolated peripheral blood monocytes, CSF-1 or -2 stimulated differentiating macrophages after 5 days as well as fully differentiated M1 and M2 macrophages after 7 days. Equal loading was assured by measurement of the housekeeping gene β actin. ...View More
Ebert, R., Cumbana, R., Lehmann, C., Kutzner, L., Toewe, A., Ferreirós, N., ... & Kahnt, A. S. (2020). Long-term stimulation of toll-like receptor-2 and-4 upregulates 5-LO and 15-LO-2 expression thereby inducing a lipid mediator shift in human monocyte-derived macrophages. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 1865(9), 158702.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
D9
Antibody Isotype
IgG1, κ
Application
IF, ELISA, IHC, WB, IP

Basic Information

Immunogen
Amino acids 423-452 within an internal region of 15-LO2 of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000
IHC1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
arachidonate 15-lipoxygenase, type B
Introduction
ALOX15B is a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. This gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described.
Entrez Gene ID
247
UniProt ID
O15296
Alternative Names
15-LOX-2
Function
Isoform A: Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids (PUFAs) generating a spectrum of bioactive lipid mediators (PubMed:9177185, PubMed:10625675, PubMed:12704195, PubMed:17493578, PubMed:18311922, PubMed:24282679, PubMed:10542053, PubMed:24497644, PubMed:32404334) (Probable). It inserts peroxyl groups at C15 of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) producing (15S)-hydroperoxyeicosatetraenoate/(15S)-HPETE (PubMed:17493578, PubMed:12704195, PubMed:24282679, PubMed:9177185, PubMed:11956198, PubMed:10625675, PubMed:24497644) (Probable). Also peroxidizes linoleate ((9Z,12Z)-octadecadienoate) to 13-hydroperoxyoctadecadienoate/13-HPODE (Probable) (PubMed:10542053, PubMed:27435673). Oxygenates arachidonyl derivatives such as 2-arachidonoylglycerol (2-AG) leading to the production and extracellular release of 15-hydroxyeicosatetraenoyl glycerol (15-HETE-G) that acts as a peroxisome proliferator-activated receptor alpha agonist (PubMed:18311922, PubMed:17493578, PubMed:11956198). Has the ability to efficiently class-switch ALOX5 pro-inflammatory mediators into anti-inflammatory intermediates (PubMed:27145229). Participates in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs) resolvin D5 ((7S,17S)-diHPDHA), which can actively downregulate the immune response and have anti-aggregation properties with platelets (PubMed:32404334). In addition to free PUFAs hydrolyzed from phospholipids, it directly oxidizes PUFAs esterified to membrane-bound phospholipids (PubMed:27435673). Has no detectable 8S-lipoxygenase activity on arachidonate but reacts with (8S)-HPETE to produce (8S,15S)-diHPETE (Probable). May regulate progression through the cell cycle and cell proliferation (PubMed:12704195, PubMed:11839751). May also regulate cytokine secretion by macrophages and therefore play a role in the immune response (PubMed:18067895). May also regulate macrophage differentiation into proatherogenic foam cells (PubMed:22912809).
Isoform B: Does not convert arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid/(15S)-HPETE.
Biological Process
Apoptotic process Source: UniProtKB
Arachidonic acid metabolic process Source: UniProtKB
Cannabinoid biosynthetic process Source: UniProtKB
Endocannabinoid signaling pathway Source: UniProtKB
Hepoxilin biosynthetic process Source: UniProtKB
Linoleic acid metabolic process Source: GO_Central
Lipid metabolic process Source: UniProtKB
Lipid oxidation Source: GO_Central
Lipoxin A4 biosynthetic process Source: UniProtKB
Lipoxygenase pathway Source: GO_Central
Negative regulation of cell cycle Source: UniProtKB
Negative regulation of cell migration Source: UniProtKB
Negative regulation of cell population proliferation Source: UniProtKB
Negative regulation of growth Source: UniProtKB
Phospholipid metabolic process Source: UniProtKB
Positive regulation of chemokine production Source: UniProtKB
Positive regulation of keratinocyte differentiation Source: Ensembl
Positive regulation of macrophage derived foam cell differentiation Source: UniProtKB
Positive regulation of peroxisome proliferator activated receptor signaling pathway Source: Ensembl
Prostate gland development Source: UniProtKB
Regulation of epithelial cell differentiation
Cellular Location
Isoform A: Nucleus. Other isoforms are excluded from the nucleus. Cytosol; Cell membrane; Cytoskeleton; Membrane; Adherens junction; Focal adhesion. Predominantly cytosolic; becomes enriched at membranes upon calcium binding.
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For research use only. Not intended for any clinical use.

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