Sign in or Register   Sign in or Register
  |  

Rabbit Anti-ALYREF Recombinant Antibody (D3R4R) (CBMAB-CP2753-LY)

The product is antibody recognizes ALYREF. The antibody D3R4R immunoassay techniques such as: WB,IP,IF (ICC).
See all ALYREF antibodies
Published Data
Rabbit Anti-ALYREF Recombinant Antibody (D3R4R) in WB
Western blotting showing ALYREF expression in 10 pairs of UCB and adjacent non-neoplastic tissues. The expression was normalized by α-tubulin expression. T, tumor tissue; N, nonneoplastic bladder tissues. ...View More
Wang, N., Chen, R. X., Deng, M. H., Wei, W. S., Zhou, Z. H., Ning, K., ... & Zhou, F. J. (2023). m5C-dependent cross-regulation between nuclear reader ALYREF and writer NSUN2 promotes urothelial bladder cancer malignancy through facilitating RABL6/TK1 mRNAs splicing and stabilization. Cell Death & Disease, 14(2), 139.
Rabbit Anti-ALYREF Recombinant Antibody (D3R4R) in IHC
Representative Hematoxylin-eosin staining images in different groups of orthotopic xenograft models. Scale bars, 100 μm. ...View More
Wang, N., Chen, R. X., Deng, M. H., Wei, W. S., Zhou, Z. H., Ning, K., ... & Zhou, F. J. (2023). m5C-dependent cross-regulation between nuclear reader ALYREF and writer NSUN2 promotes urothelial bladder cancer malignancy through facilitating RABL6/TK1 mRNAs splicing and stabilization. Cell Death & Disease, 14(2), 139.

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat, Monkey
Clone
D3R4R
Antibody Isotype
IgG
Application
WB, IP, IF (ICC)

Basic Information

Immunogen
Synthetic peptide corresponding to residues surrounding Ala192 of human THOC4/ALY protein.
Host Species
Rabbit
Specificity
Human, Mouse, Rat, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IP1:50
IF(ICC)1:400

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
10 mM HEPES, 150 mM NaCl, pH 7.5, 100 μg/mL BSA, 50% Glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Aly/REF Export Factor
Introduction
The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human10189
Mouse21681
Rat690585
Monkey714852
UniProt ID
HumanQ86V81
MouseO08583
RatD3ZXH7
MonkeyA0A2K5U747
Alternative Names
Aly/REF Export Factor; Transcriptional Coactivator Aly/REF; BZIP-Enhancing Factor BEF; Ally Of AML-1 And LEF-1; THO Complex 4; THOC4; Tho4;
Function
Export adapter involved in nuclear export of spliced and unspliced mRNA. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NFX1 pathway) (PubMed:15833825, PubMed:15998806, PubMed:17190602, PubMed:11707413, PubMed:11675789, PubMed:11979277, PubMed:18364396, PubMed:22144908, PubMed:22893130, PubMed:23222130, PubMed:25662211). Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA (PubMed:15833825, PubMed:15998806, PubMed:17190602). TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm (PubMed:15833825, PubMed:15998806, PubMed:17190602). TREX recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1 (PubMed:15833825, PubMed:15998806, PubMed:17190602). The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production; ALYREF/THOC4 mediates the recruitment of the TREX complex to the intronless viral mRNA (PubMed:18974867). Required for TREX complex assembly and for linking DDX39B to the cap-binding complex (CBC) (PubMed:15998806, PubMed:17984224). In conjunction with THOC5 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim (PubMed:19165146). Involved in the nuclear export of intronless mRNA; proposed to be recruited to intronless mRNA by ATP-bound DDX39B. Involved in transcription elongation and genome stability (PubMed:12438613, PubMed:17984224). Involved in mRNA export of C5-methylcytosine (m5C)-containing mRNAs: specifically recognizes and binds m5C mRNAs and mediates their nucleo-cytoplasmic shuttling (PubMed:28418038).
Acts as chaperone and promotes the dimerization of transcription factors containing basic leucine zipper (bZIP) domains and thereby promotes transcriptional activation.
Biological Process
mRNA 3'-end processing Source: Reactome
mRNA export from nucleus Source: UniProtKB
mRNA splicing, via spliceosome Source: Reactome
Osteoblast differentiation Source: UniProtKB
Positive regulation of DNA-templated transcription, elongation Source: UniProtKB
Regulation of DNA recombination Source: UniProtKB
Replication fork processing Source: UniProtKB
RNA export from nucleus Source: UniProtKB
Viral mRNA export from host cell nucleus Source: UniProtKB
Viral process Source: UniProtKB-KW
Cellular Location
Nucleus; Nucleus speckle; Cytoplasm. Colocalizes with the core EJC, ALYREF/THOC4, NXF1 and DDX39B in the nucleus and nuclear speckles. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA (PubMed:19324961). Localizes to regions surrounding nuclear speckles known as perispeckles in which TREX complex assembly seems to occur (PubMed:23826332).
PTM
Arg-204 is dimethylated, probably to asymmetric dimethylarginine. Arginine methylation reduces RNA binding.2 Publications
Citrullinated by PADI4.
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Rabbit Anti-ALYREF Recombinant Antibody (D3R4R)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare