Mouse Anti-ANAPC1 Recombinant Antibody (CBT2905) (V2LY-0625-LY725)

Name: Mouse Anti-ANAPC1 Recombinant Antibody (CBT2905)
SKU: V2LY-0625-LY725
Rating: 5 (1 reviews)

See all ANAPC1 antibodies

Compare Online Inquiry

Tested Data

Mouse Anti-ANAPC1 Recombinant Antibody (CBT2905) (V2LY-0625-LY725) in ELISA

Mouse Anti-ANAPC1 Recombinant Antibody (CBT2905) (V2LY-0625-LY725) in WB

Mouse Anti-ANAPC1 Recombinant Antibody (CBT2905) (V2LY-0625-LY725) in IF

Mouse Anti-ANAPC1 Recombinant Antibody (CBT2905) (V2LY-0625-LY725) in FC

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT2905

Application

ICC, FC

Immunogen

Purified recombinant fragment of human ANAPC1 (AA: 12-155) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
ICC	1:100-1:500
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Anaphase Promoting Complex Subunit 1

Entrez Gene ID

64682

UniProt ID

Q9H1A4

Function

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Biological Process

Anaphase-promoting complex-dependent catabolic process Source: GO_Central
Cell division Source: UniProtKB-KW
Metaphase/anaphase transition of mitotic cell cycle Source: GO_Central
Protein K11-linked ubiquitination Source: UniProtKB
Regulation of exit from mitosis Source: Reactome
Regulation of mitotic cell cycle phase transition Source: Reactome
Ubiquitin-dependent protein catabolic process Source: Reactome

Cellular Location

Cytosol; Anaphase-promoting complex; Nucleoplasm

Involvement in disease

Rothmund-Thomson syndrome 1 (RTS1): An autosomal recessive disorder characterized by sparse hair, bilateral juvenile cataracts, and poikiloderma, a genodermatosis presenting with mottled pigmentation, telangiectasia and epidermal atrophy. Additional features are short stature, dystrophic and thin nails, and genital, skeletal and dental abnormalities. RTS1 is not associated with an increased risk of cancer.

PTM

Phosphorylated. Phosphorylation on Ser-355 ^oCcurs specifically during mitosis.

Zirn, B., Bernbeck, U., Alt, K., Oeffner, F., Gerhardinger, A., & Has, C. (2021). Rothmund–Thomson syndrome type 1 caused by biallelic ANAPC1 gene mutations. Skin Health and Disease, 1(1), e12.

Ajeawung, N. F., Nguyen, T. T. M., Lu, L., Kucharski, T. J., Rousseau, J., Molidperee, S., ... & Campeau, P. M. (2019). Mutations in ANAPC1, encoding a scaffold subunit of the anaphase-promoting complex, cause Rothmund-Thomson syndrome type 1. The American Journal of Human Genetics, 105(3), 625-630.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-ANAPC1 Recombinant Antibody (CBT2905)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-ANAPC1 Recombinant Antibody (CBT3511) (CAT#: V2LY-0625-LY624)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry