Mouse Anti-ANAPC11 Recombinant Antibody (1B4-1A4) (CBMAB-A0344-LY)

Name: Mouse Anti-ANAPC11 Recombinant Antibody (1B4-1A4)
SKU: CBMAB-A0344-LY
Rating: 5 (1 reviews)

See all ANAPC11 antibodies

Compare Online Inquiry

Published Data

Mouse Anti-ANAPC11 Recombinant Antibody (1B4-1A4) in IP

Representative western blot analysis of the indicated proteins in whole-cell extracts (WCE; input; left) and of proteins co-immunoprecipitated (right) with RECQ4 using a rabbit anti-RECQ4 antibody at the indicated time points post-nocodazole (Noc) release. ...View More

Xu, X., Chang, C. W., Li, M., Omabe, K., Le, N., Chen, Y. H., ... & Liu, Y. (2023). DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation. Nature Communications, 14(1), 1233.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1B4-1A4

Application

WB, ELISA, IP

Immunogen

Amino acids 1-54 representing full length APC11 of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

anaphase promoting complex subunit 11

Entrez Gene ID

51529

UniProt ID

Q9NYG5

Alternative Names

APC11; Apc11p; HSPC214; MGC882

Function

Together with the cullin protein ANAPC2, constitutes the catalytic component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. May recruit the E2 ubiquitin-conjugating enzymes to the complex.

Biological Process

Anaphase-promoting complex-dependent catabolic process Source: Reactome
Cell division Source: UniProtKB-KW
Mitotic cell cycle Source: UniProtKB
Positive regulation of mitotic metaphase/anaphase transition Source: GO_Central
Protein K11-linked ubiquitination Source: UniProtKB
Protein ubiquitination Source: UniProtKB
Regulation of exit from mitosis Source: Reactome
Regulation of mitotic cell cycle phase transition Source: Reactome
Ubiquitin-dependent protein catabolic process Source: GO_Central

Cellular Location

Cytoplasm; Nucleus

PTM

Auto-ubiquitinated.

Zhang, Y., & Zhou, H. (2020). LncRNA BCAR4 promotes liver cancer progression by upregulating ANAPC11 expression through sponging miR‑1261. International journal of molecular medicine, 46(1), 159-166.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-ANAPC11 Recombinant Antibody (1B4-1A4)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-ANAPC11 Recombinant Antibody (CBT3936) (CAT#: V2LY-0625-LY575)

Mouse Anti-ANAPC11 Recombinant Antibody (CBT4475) (CAT#: V2LY-0625-LY576)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry