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Mouse Anti-ANAPC2 Recombinant Antibody (CBYC-A574) (CBMAB-A2642-YC)

Provided herein is a Mouse monoclonal antibody against Human Anaphase Promoting Complex Subunit 2. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all ANAPC2 antibodies
Published Data
Mouse Anti-ANAPC2 Recombinant Antibody (CBYC-A574) in IP
To investigate whether OLC1 may bind directly to the components of the APC/c, KYSE150/GFP-OLC1 cells were collected, lysed and subjected to IP. The presence of actin, OLC1 and APC2 in the immunocomplex were verified. ...View More
Zhang, X., Chen, W., Yin, N., Dong, L., Fu, M., Zhan, Q., & Tong, T. (2019). Regulation of OLC1 protein expression by the anaphase‑promoting complex. Oncology Letters, 17(3), 2639-2646.

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYC-A574
Antibody Isotype
IgG1, κ
Application
ELISA, WB, IP

Basic Information

Immunogen
Amino acids 716-822 representing partial length ANAPC2 of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ANAPC2
Introduction
A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the
Entrez Gene ID
29882
UniProt ID
Q9UJX6
Alternative Names
Anaphase Promoting Complex Subunit 2; Cyclosome Subunit 2; APC2; Anaphase-Promoting Complex Subunit 2; KIAA1406;
Function
Together with the RING-H2 protein ANAPC11, constitutes the catalytic component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 drives presynaptic differentiation.
Biological Process
Anaphase-promoting complex-dependent catabolic process Source: Reactome
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Negative regulation of gene expression Source: Ensembl
Positive regulation of axon extension Source: Ensembl
Positive regulation of dendrite morphogenesis Source: Ensembl
Positive regulation of synapse maturation Source: UniProtKB
Positive regulation of synaptic plasticity Source: UniProtKB
Protein K11-linked ubiquitination Source: UniProtKB
Regulation of exit from mitosis Source: Reactome
Regulation of mitotic cell cycle phase transition Source: Reactome
Ubiquitin-dependent protein catabolic process Source: Reactome
Cellular Location
Cytosol; Anaphase-promoting complex; Nucleoplasm
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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