Mouse Anti-ANAPC2 Recombinant Antibody (CBYC-A574) (CBMAB-A2642-YC)

Name: Mouse Anti-ANAPC2 Recombinant Antibody (CBYC-A574)
SKU: CBMAB-A2642-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ANAPC2 Recombinant Antibody (CBYC-A574) in IP

To investigate whether OLC1 may bind directly to the components of the APC/c, KYSE150/GFP-OLC1 cells were collected, lysed and subjected to IP. The presence of actin, OLC1 and APC2 in the immunocomplex were verified. ...View More

Zhang, X., Chen, W., Yin, N., Dong, L., Fu, M., Zhan, Q., & Tong, T. (2019). Regulation of OLC1 protein expression by the anaphase‑promoting complex. Oncology Letters, 17(3), 2639-2646.

Datasheet

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Basic Information

Host Animal

Mouse

Clone

CBYC-A574

Application

ELISA, WB, IP

Immunogen

Amino acids 716-822 representing partial length ANAPC2 of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ANAPC2

Introduction

A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the

Entrez Gene ID

29882

UniProt ID

Q9UJX6

Alternative Names

Anaphase Promoting Complex Subunit 2; Cyclosome Subunit 2; APC2; Anaphase-Promoting Complex Subunit 2; KIAA1406;

Function

Together with the RING-H2 protein ANAPC11, constitutes the catalytic component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 drives presynaptic differentiation.

Biological Process

Anaphase-promoting complex-dependent catabolic process Source: Reactome
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Negative regulation of gene expression Source: Ensembl
Positive regulation of axon extension Source: Ensembl
Positive regulation of dendrite morphogenesis Source: Ensembl
Positive regulation of synapse maturation Source: UniProtKB
Positive regulation of synaptic plasticity Source: UniProtKB
Protein K11-linked ubiquitination Source: UniProtKB
Regulation of exit from mitosis Source: Reactome
Regulation of mitotic cell cycle phase transition Source: Reactome
Ubiquitin-dependent protein catabolic process Source: Reactome

Cellular Location

Cytosol; Anaphase-promoting complex; Nucleoplasm

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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