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Mouse Anti-ANAPC4 Recombinant Antibody (CIV1.1) (CBMAB-XB0039-YC)

Provided herein is a Mouse Recombinant Antibody against Anaphase Promoting Complex Subunit 4. The antibody can be used for immunoassay techniques, such as IP, WB.
See all ANAPC4 antibodies
Published Data
Mouse Anti-ANAPC4 Recombinant Antibody (CIV1.1) in IP
Whole-cell lysates from G1-synchronized NSTCs and CSCs from GBM 3832 (C), 3691 (D), and 08-387 (E) were probed for APC4 and CDH1. b-Actin served as a loading control. The APC/C holoenzyme was immunoprecipitated from G1-synchronized NSTCs and CSCs from GBM 3832 using anti-CDC27 (F) or anti-APC4 (G) and from 3691 using anti-APC4 (H). Resulting immunoprecipitates were probed for CDC27 or APC4 and CDH1 (top two panels). Control lane, anti-CDC27 or anti-APC4 antibody and beads only. Bound CDH1 relative to CDC27 or APC4 as an indication of active APC/C was quantified and resulting fold change graphed (normalized to NSTCs). n ¼ 3 biological replicates; error bars, SD; , P < 0.05;  , P < 0.01; , P < 0.001. Bottom two panels represent inputs for the CDC27 or APC4 immunoprecipitation. b-Actin or a-tubulin served as loading controls. ...View More
De, K., Grubb, T. M., Zalenski, A. A., Pfaff, K. E., Pal, D., Majumder, S., ... & Venere, M. (2019). Hyperphosphorylation of CDH1 in glioblastoma cancer stem cells attenuates APC/CCDH1 activity and pharmacologic inhibition of APC/CCDH1/CDC20 compromises viability. Molecular Cancer Research, 17(7), 1519-1530.

Summary

Host Animal
Mouse
Specificity
Human
Clone
CIV1.1
Antibody Isotype
IgG
Application
IP, WB

Basic Information

Immunogen
C-terminus of APC4
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:500
IP1:10-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.02% sodium azide
Concentration
1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Anaphase Promoting Complex Subunit 4
Introduction
A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The exact function of this gene product is not known.
Entrez Gene ID
29945
UniProt ID
Q9UJX5
Alternative Names
Anaphase Promoting Complex Subunit 4; Cyclosome Subunit 4; APC4; Anaphase-Promoting Complex Subunit 4;
Function
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Biological Process
Anaphase-promoting complex-dependent catabolic process Source: GO_Central
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Positive regulation of mitotic metaphase/anaphase transition Source: GO_Central
Protein K11-linked ubiquitination Source: UniProtKB
Regulation of exit from mitosis Source: Reactome
Regulation of mitotic cell cycle phase transition Source: Reactome
Ubiquitin-dependent protein catabolic process Source: Reactome
Cellular Location
Nucleus
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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