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Mouse Anti-ANAPC5 Recombinant Antibody (APC5#4) (CBMAB-A2645-YC)

Provided herein is a Mouse monoclonal antibody against Human Anaphase Promoting Complex Subunit 5. The antibody can be used for immunoassay techniques, such as WB.
See all ANAPC5 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
APC5#4
Antibody Isotype
IgG2b, κ
Application
ELISA, WB, IP, IF

Basic Information

Immunogen
Amino acids 1-300 of APC5 of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Anaphase Promoting Complex Subunit 5
Introduction
ANAPC5 is a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S p
Entrez Gene ID
51433
UniProt ID
Q9UJX4
Alternative Names
Anaphase Promoting Complex Subunit 5; Cyclosome Subunit 5; APC5; Anaphase-Promoting Complex Subunit 5;
Function
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Biological Process
Anaphase-promoting complex-dependent catabolic process Source: GO_Central
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Positive regulation of mitotic metaphase/anaphase transition Source: GO_Central
protein K11-linked ubiquitination Source: UniProtKB
Regulation of exit from mitosis Source: Reactome
Regulation of mitotic cell cycle phase transition Source: Reactome
Ubiquitin-dependent protein catabolic process Source: Reactome
Cellular Location
Nucleus; Spindle
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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