Mouse Anti-ANGPTL4 Recombinant Antibody (C7) (CBMAB-A2722-YC)

Name: Mouse Anti-ANGPTL4 Recombinant Antibody (C7)
SKU: CBMAB-A2722-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ANGPTL4 Recombinant Antibody (C7) in WB

Angiopoietin-like 4 (ANGPTL4) mRNA and protein levels were determined in the shRNA-TNMD and shRNA-control adipocytes. Protein levels in cell lysates were analyzed by Western blotting using specifc antibodies against ANGPTL4, normalized to the internal control (α-tubulin), and expressed as fold-change; the lower section shows a representative crop blot. ...View More

Ruiz-Ojeda, F. J., Anguita-Ruiz, A., Rupérez, A. I., Gomez-Llorente, C., Olza, J., Vázquez-Cobela, R., ... & Aguilera, C. M. (2019). Effects of X-chromosome Tenomodulin genetic variants on obesity in a children's cohort and implications of the gene in adipocyte metabolism. Scientific Reports, 9(1), 3979.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

Application

ELISA, ICC, IHC, WB

Immunogen

Amino acids 51-250 mapping near the N-terminus of Angptl4 of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

angiopoietin-like 4

Introduction

ANGPTL4 is a glycosylated, secreted protein containing a C-terminal fibrinogen domain. The encoded protein is induced by peroxisome proliferation activators and functions as a serum hormone that regulates glucose homeostasis, lipid metabolism, and insulin

Entrez Gene ID

362850

UniProt ID

Q6TMA8

Alternative Names

Angiopoietin Like 4; Hepatic Fibrinogen/Angiopoietin-Related Protein; Peroxisome Proliferator-Activated Receptor (PPAR) Gamma Induced Angiopoietin-Related Protein; Hepatic Angiopoietin-Related Protein; PPARG Angiopoietin Related Protein; Fasting-Induced A

Function

Mediates inactivation of the lipoprotein lipase LPL, and thereby plays a role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism (PubMed:19270337, PubMed:21398697, PubMed:27929370, PubMed:29899144). May also play a role in regulating glucose homeostasis and insulin sensitivity (Probable). Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage (PubMed:14583458, PubMed:17068295). Upon heterologous expression, inhibits the adhesion of endothelial cell to the extracellular matrix (ECM), and inhibits the reorganization of the actin cytoskeleton, formation of actin stress fibers and focal adhesions in endothelial cells that have adhered to ANGPTL4-containing ECM (in vitro) (PubMed:17068295). Depending on context, may modulate tumor-related angiogenesis (By similarity).
ANGPTL4 N-terminal chain: Mediates inactivation of the lipoprotein lipase LPL, and thereby plays an important role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism (PubMed:19270337, PubMed:21398697, PubMed:27929370, PubMed:29899144). Has higher activity in LPL inactivation than the uncleaved protein (PubMed:19270337, PubMed:21398697).

Biological Process

Angiogenesis Source: UniProtKB-KW
Lipid metabolic process Source: UniProtKB-KW
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of endothelial cell apoptotic process Source: Ensembl
Negative regulation of lipoprotein lipase activity Source: BHF-UCL
Positive regulation of angiogenesis Source: UniProtKB
Protein unfolding Source: ARUK-UCL
Regulation of metabolic process Source: Reactome
Response to hypoxia Source: UniProtKB
Triglyceride homeostasis Source: BHF-UCL

Cellular Location

Secreted; Extracellular matrix. The unprocessed form interacts with the extracellular matrix (PubMed:17068295, PubMed:21398697). This may constitute a dynamic reservoir, a regulatory mechanism of the bioavailability of ANGPTL4 (Probable).

PTM

N-glycosylated.
ANGPTL4 N-terminal chain: Forms disulfide-linked dimers and tetramers.
Cleaved into a smaller N-terminal chain and a larger chain that contains the fibrinogen C-terminal domain; both cleaved and uncleaved forms are detected in the extracellular space. The cleaved form is not present within the cell.

Sodhi, A., Ma, T., Menon, D., Deshpande, M., Jee, K., Dinabandhu, A., ... & Montaner, S. (2019). Angiopoietin-like 4 binds neuropilins and cooperates with VEGF to induce diabetic macular edema. The Journal of clinical investigation, 129(11), 4593-4608.

Theofilatos, D., Fotakis, P., Valanti, E., Sanoudou, D., Zannis, V., & Kardassis, D. (2018). HDL-apoA-I induces the expression of angiopoietin like 4 (ANGPTL4) in endothelial cells via a PI3K/AKT/FOXO1 signaling pathway. Metabolism, 87, 36-47.

Dijk, W., Ruppert, P. M., Oost, L. J., & Kersten, S. (2018). Angiopoietin-like 4 promotes the intracellular cleavage of lipoprotein lipase by PCSK3/furin in adipocytes. Journal of Biological Chemistry, 293(36), 14134-14145.

Zhang, T., Kastrenopoulou, A., Larrouture, Q., Athanasou, N. A., & Knowles, H. J. (2018). Angiopoietin-like 4 promotes osteosarcoma cell proliferation and migration and stimulates osteoclastogenesis. BMC cancer, 18(1), 1-10.

Dijk, W., Schutte, S., Aarts, E. O., Janssen, I. M., Afman, L., & Kersten, S. (2018). Regulation of angiopoietin-like 4 and lipoprotein lipase in human adipose tissue. Journal of clinical lipidology, 12(3), 773-783.

Janssen, A. W., Katiraei, S., Bartosinska, B., Eberhard, D., van Dijk, K. W., & Kersten, S. (2018). Loss of angiopoietin-like 4 (ANGPTL4) in mice with diet-induced obesity uncouples visceral obesity from glucose intolerance partly via the gut microbiota. Diabetologia, 61(6), 1447-1458.

Yang, X., Cheng, Y., & Su, G. (2018). A review of the multifunctionality of angiopoietin-like 4 in eye disease. Bioscience reports, 38(5).

Cushing, E. M., Chi, X., Sylvers, K. L., Shetty, S. K., Potthoff, M. J., & Davies, B. S. (2017). Angiopoietin-like 4 directs uptake of dietary fat away from adipose during fasting. Molecular metabolism, 6(8), 809-818.

McQueen, A. E., Kanamaluru, D., Yan, K., Gray, N. E., Wu, L., Li, M. L., ... & Wang, J. C. (2017). The C-terminal fibrinogen-like domain of angiopoietin-like 4 stimulates adipose tissue lipolysis and promotes energy expenditure. Journal of Biological Chemistry, 292(39), 16122-16134.

Cara-Fuentes, G., Segarra, A., Silva-Sanchez, C., Wang, H., Lanaspa, M. A., Johnson, R. J., & Garin, E. H. (2017). Angiopoietin-like-4 and minimal change disease. PloS one, 12(4), e0176198.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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