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Mouse Anti-ANK1 Recombinant Antibody (H4) (CBMAB-A2747-YC)

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Basic Information

Host Animal
Mouse
Clone
H4
Application
WB, IP, IF, IHC-P, ELISA
Immunogen
Amino acids 820-952 mapping within an internal region of Ankyrin-1 isoform 9 of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
Ankyrin 1
Introduction
Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane
Entrez Gene ID
Human286
Pig100157679
Rat306570
Cattle353108
Dog100686820
Rabbit100356919
UniProt ID
HumanP16157
PigF1SE30
RatD3Z9Z0
DogF1PRC8
RabbitG1TG85
Alternative Names
Ankyrin 1; Ankyrin 1, Erythrocytic; Erythrocyte Ankyrin; Ankyrin-R; ANK-1; ANK; Ankyrin-1; SPH1; SPH2;
Function
Attaches integral membrane proteins to cytoskeletal elements; binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin. Erythrocyte ankyrins also link spectrin (beta chain) to the cytoplasmic domain of the erythrocytes anion exchange protein; they retain most or all of these binding functions.
Isoform Mu17: Together with obscurin in skeletal muscle may provide a molecular link between the sarcoplasmic reticulum and myofibrils.
Biological Process
Cytoskeleton organization Source: UniProtKB
Endoplasmic reticulum to Golgi vesicle-mediated transport Source: BHF-UCL
Exocytosis Source: UniProtKB
Maintenance of epithelial cell apical/basal polarity Source: UniProtKB
Positive regulation of organelle organization Source: Ensembl
Protein localization to plasma membrane Source: BHF-UCL
Signal transduction Source: InterPro
Cellular Location
Isoform Er1: Cytoskeleton. Probably the other erythrocyte (Er) isoforms, are located near the surface of erythrocytic plasma membrane.
Isoform Mu17: Membrane; M line. Colocalizes with OBSCN isoform 3/obscurin at the M line in differentiated skeletal muscle cells.
Isoform Mu18: Sarcoplasmic reticulum
Isoform Mu19: Sarcoplasmic reticulum
Isoform Mu20: Sarcoplasmic reticulum
Involvement in disease
Spherocytosis 1 (SPH1): A form of spherocytosis, a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. SPH1 is characterized by severe hemolytic anemia. Inheritance can be autosomal dominant or autosomal recessive. Patients with homozygous mutations have a more severe disorder.
PTM
Regulated by phosphorylation.
Palmitoylated.
Hydroxylated by HIF1AN at several asparagine and 1 aspartate residue within ANK repeat region. Hydroxylation seems to increase the conformational stability of this region and may also modulate protein-protein interactions mediated by the ANK repeat region.
(Microbial infection) Probably cleaved by P.falciparum SERA6; the cleavage probably causes the disruption of the actin cytoskeleton and the rupture of the erythrocyte cell membrane releasing the merozoites.

Hu, J., Gao, X., Shi, B., Chen, H., Zhao, Z., Wang, J., ... & Luo, Y. (2021). Sequence and haplotypes of ankyrin 1 gene (ANK1) and their association with carcass and meat quality traits in yak. Mammalian Genome, 32(2), 104-114.

Lazzareschi, I., Curatola, A., Pedicelli, C., Castiglia, D., Buonsenso, D., Gatto, A., ... & Valentini, P. (2019). A previously unrecognized Ankyrin‐1 mutation associated with Hereditary Spherocytosis in an Italian family. European journal of haematology, 103(5), 523-526.

Huang, H. M., Bauer, D. C., Lelliott, P. M., Dixon, M. W., Tilley, L., McMorran, B. J., ... & Burgio, G. (2017). Ankyrin-1 gene exhibits allelic heterogeneity in conferring protection against malaria. G3: Genes, Genomes, Genetics, 7(9), 3133-3144.

Liu, S., Jiang, H., Huang, L. Y., & Li, D. Z. (2017). A de novo ankyrin mutation (ANK1 Q109X) causing severe hereditary spherocytosis from preterm neonatal period. Annals of hematology, 96(6), 1067.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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