Mouse Anti-ANKRD11 Recombinant Antibody (CBYC-A606) (CBMAB-A2774-YC)

Name: Mouse Anti-ANKRD11 Recombinant Antibody (CBYC-A606)
SKU: CBMAB-A2774-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ANKRD11 Recombinant Antibody (CBYC-A606) in IF

Fluorescence in situ hybridization (FISH) analysis for XCE17 shows aneuploid cells in shANCO1 DCIS cell lines. Images were taken under 10× ocular and 100× objective lenses. ...View More

Yuan, M., Barefoot, M. E., Peterson, K., Campbell, M. J., Blancato, J. K., Chen, M., ... & Sharif, G. M. (2023). Loss of ANCO1 Expression Regulates Chromatin Accessibility and Drives Progression of Early-Stage Triple-Negative Breast Cancer. International Journal of Molecular Sciences, 24(14), 11505.

Mouse Anti-ANKRD11 Recombinant Antibody (CBYC-A606) in WB

shAnkrd11 knocks down endogenous ANKRD11 in cortical neurons. Isolated cortical cells from E14.5 mice were transfected with non-silencing shRNA (control) or shAnkrd11, and cultured for 7 days. Cellular lysates were then subjected to Western blotting. ...View More

Ka, M., & Kim, W. Y. (2018). ANKRD11 associated with intellectual disability and autism regulates dendrite differentiation via the BDNF/TrkB signaling pathway. Neurobiology of disease, 111, 138-152.

Mouse Anti-ANKRD11 Recombinant Antibody (CBYC-A606) in IF

Ankrd11 knockdown induces multiple shorter leading processes in radially migrating pyramidal neurons. A construct encoding control or shAnkrd11 was electroporated in utero into E14.5 mice. Migrating neurons expressing GFP were visualized at E17.5. Scale bar: 25 μm. ...View More

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYC-A606

Application

IP, WB

Immunogen

Recombinant protein corresponding to an internal region of ANKRD11 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Ankyrin Repeat Domain 11

Introduction

ANKRD11 is an ankryin repeat domain-containing protein. ANKRD11 inhibits ligand-dependent activation of transcription. Mutations in ANKRD11 have been associated with KBG syndrome, which is characterized by macrodontia, distinctive craniofacial features, s

Entrez Gene ID

Human	29123
Mouse	77087

UniProt ID

Human	Q6UB99
Mouse	E9Q4F7

Alternative Names

Ankyrin Repeat Domain 11; Ankyrin Repeat-Containing Cofactor 1; ANCO1; Truncated Ankyrin Repeat Domain 11 Aberrant Transcript 1; Truncated Ankyrin Repeat Domain 11 Aberrant Transcript 2; Nasopharyngeal Carcinoma Susceptibility Protein;

Function

Chromatin regulator which modulates histone acetylation and gene expression in neural precursor cells (By similarity). May recruit histone deacetylases (HDACs) to the p160 coactivators/nuclear receptor complex to inhibit ligand-dependent transactivation (PubMed:15184363). Has a role in proliferation and development of cortical neural precursors (PubMed:25556659). May also regulate bone homeostasis (By similarity).

Biological Process

Anatomical structure morphogenesis Source: GO_Central
Face morphogenesis Source: MGI
Odontogenesis of dentin-containing tooth Source: MGI
Skeletal system morphogenesis Source: MGI

Cellular Location

Nucleus. Localizes to chromatin during prometaphase.

Involvement in disease

KBG syndrome (KBGS): A syndrome characterized by macrodontia of the upper central incisors, distinctive craniofacial findings, short stature, skeletal anomalies, and neurologic involvement that includes global developmental delay, seizures, and intellectual disability.

PTM

Subject to proteasomal degradation which is probably essential to regulate its activity.

Parenti, I., Mallozzi, M. B., Hüning, I., Gervasini, C., Kuechler, A., Agolini, E., ... & Kaiser, F. J. (2021). ANKRD11 variants: KBG syndrome and beyond. Clinical Genetics.

Chen, J., Xia, Z., Zhou, Y., Ma, X., Wang, X., & Guo, Q. (2021). A de novo frameshift variant of ANKRD11 (c. 1366_1367dup) in a Chinese patient with KBG syndrome. BMC medical genomics, 14(1), 1-7.

Ilaria, P., Mallozzi, M. B., Irina, H., Gervasini, C. C. G., Alma, K., Emanuele, A., ... & Kaiser, F. J. (2021). ANKRD11 variants: KBG syndrome and beyond.

Roth, D. M., Baddam, P., Lin, H., Vidal-García, M., Aponte, J. D., De Souza, S. T., ... & Voronova, A. (2021). The Chromatin Regulator Ankrd11 Controls Palate and Cranial Bone Development. Frontiers in cell and developmental biology, 9, 985.

Kim, S. J., Yang, A., Park, J. S., Kwon, D. G., Lee, J. S., Kwon, Y. S., & Lee, J. E. (2020). Two novel mutations of ANKRD11 gene and wide clinical spectrum in KBG syndrome: case reports and literature review. Frontiers in Genetics, 11.

Seo, G. H., Oh, A., Kang, M., Kim, E. N., Jang, J. H., Kim, D. Y., ... & Lee, B. H. (2019). An ANKRD11 exonic deletion accompanied by a congenital megacolon in an infant with KBG syndrome. Journal of Genetic Medicine, 16(1), 39-42.

Alves, R. M., Uva, P., Veiga, M. F., Oppo, M., Zschaber, F. C., Porcu, G., ... & Crisponi, L. (2019). Novel ANKRD11 gene mutation in an individual with a mild phenotype of KBG syndrome associated to a GEFS+ phenotypic spectrum: a case report. BMC medical genetics, 20(1), 1-7.

Morton, V. H., Quinlan‐Jones, E., Butts, N., Williams, D., Hamilton, S., Marton, T., & Morris, K. (2018). The first antenatal diagnosis of KBG syndrome: a microdeletion at chromosome 16q24. 2q24. 3 containing multiple genes including ANKRD11 associated with the disorder. Clinical case reports, 6(1), 189.

De Bernardi, M. L., Ivanovski, I., Caraffi, S. G., Maini, I., Street, M. E., Bayat, A., ... & Garavelli, L. (2018). Prominent and elongated coccyx, a new manifestation of KBG syndrome associated with novel mutation in ANKRD11. American Journal of Medical Genetics Part A, 176(9), 1991-1995.

Bianchi, P. M., Bianchi, A., Digilio, M. C., Tucci, F. M., Sitzia, E., & De Vincentiis, G. C. (2017). Audiological findings in a de novo mutation of ANKRD11 gene in KBG syndrome: Report of a case and review of the literature. International journal of pediatric otorhinolaryngology, 103, 109-112.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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