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Mouse Anti-ANKRD15 Recombinant Antibody (2B8) (CBMAB-A0366-LY)

The product is antibody recognizes ANKRD15. The antibody 2B8 immunoassay techniques such as: WB, ELISA.
See all ANKRD15 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2B8
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
ANKRD15 (AAH38116, 701 a.a. ~ 800 a.a) partial recombinant protein with GST tag.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
KN motif and ankyrin repeat domains 1
Introduction
This gene encodes a protein containing four ankyrin repeat domains in its C-terminus. The suggested role for this protein is in tumorigenesis of renal cell carcinoma. Two alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]
Entrez Gene ID
23189
UniProt ID
Q14678
Alternative Names
ANKRD15; DKFZp451G231; KANK; KIAA0172; MGC43128
Function
Involved in the control of cytoskeleton formation by regulating actin polymerization. Inhibits actin fiber formation and cell migration (PubMed:25961457). Inhibits RhoA activity; the function involves phosphorylation through PI3K/Akt signaling and may depend on the competetive interaction with 14-3-3 adapter proteins to sequester them from active complexes (PubMed:25961457). Inhibits the formation of lamellipodia but not of filopodia; the function may depend on the competetive interaction with BAIAP2 to block its association with activated RAC1 (PubMed:25961457). Inhibits fibronectin-mediated cell spreading; the function is partially mediated by BAIAP2. Inhibits neurite outgrowth. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. In the nucleus, is involved in beta-catenin-dependent activation of transcription. Potential tumor suppressor for renal cell carcinoma. Regulates Rac signaling pathways (PubMed:25961457).
Biological Process
Actin cytoskeleton organization Source: UniProtKB
Cell population proliferation Source: UniProtKB
Glomerular visceral epithelial cell migration Source: UniProtKB
Negative regulation of actin filament polymerization Source: UniProtKB
Negative regulation of cell migration Source: UniProtKB
Negative regulation of insulin receptor signaling pathway Source: UniProtKB
Negative regulation of lamellipodium morphogenesis Source: UniProtKB
Negative regulation of neuron projection development Source: UniProtKB
Negative regulation of Rho protein signal transduction Source: UniProtKB
Negative regulation of ruffle assembly Source: UniProtKB
Negative regulation of substrate adhesion-dependent cell spreading Source: UniProtKB
Positive regulation of canonical Wnt signaling pathway Source: UniProtKB
Positive regulation of Wnt signaling pathway Source: UniProtKB
Positive regulation of wound healing Source: UniProtKB
Regulation of establishment of cell polarity Source: UniProtKB
Regulation of Rho protein signal transduction Source: UniProtKB
Cellular Location
Nucleus; Cytoplasm; Ruffle membrane. Colocalizes with KIF21A in membrane ruffles (PubMed:19559006). Shuttles between the cytoplasm and nucleus (PubMed:16968744).
Isoform 1: Nucleus; Cytoplasm. Shuttles between the cytoplasm and nucleus.
Isoform 2: Nucleus; Cytoplasm. Shuttles between the cytoplasm and nucleus.
Involvement in disease
Cerebral palsy, spastic quadriplegic 2 (CPSQ2): A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest congenital hypotonia evolving over the first year to spastic quadriplegia with accompanying transient nystagmus and varying degrees of mental retardation. Neuroimaging shows brain atrophy and ventriculomegaly.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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