Mouse Anti-ANKS3 Recombinant Antibody (1B2) (CBMAB-A0371-LY)

The product is antibody recognizes ANKS3. The antibody 1B2 immunoassay techniques such as: WB, ELISA.
See all ANKS3 antibodies

Summary

Host Animal

Mouse

Specificity

Human

Clone

1B2

Antibody Isotype

IgG1, κ

Application

WB, ELISA

Basic Information

Immunogen

ANKS3 (AAH50387.1, 1 a.a. ~ 527 a.a) full-length recombinant protein with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

ankyrin repeat and sterile alpha motif domain containing 3

Entrez Gene ID

124401

UniProt ID

Q6ZW76

Alternative Names

FLJ32345; FLJ32767; KIAA1977

Function

May be involved in vasopressin signaling in the kidney.

Cellular Location

Cytoplasm; Cilium

PTM

Hydroxylated at Asn-96, most probably by HIF1AN.
Phosphorylations at Ser-5, Ser-225, Thr-319, Ser-320, Ser-368 and Ser-371 ^oCcur in a NEK7-dependent manner.
Polyubiquitinated.

References

Clark, E. (2020). In Vivo Characterisation of ANKS3-A New Candidate for Ciliopathic Disease (Doctoral dissertation).

Rothé, B., Leettola, C. N., Leal-Esteban, L., Cascio, D., Fortier, S., Isenschmid, M., ... & Constam, D. B. (2018). Crystal structure of Bicc1 SAM polymer and mapping of interactions between the ciliopathy-associated proteins Bicc1, ANKS3, and ANKS6. Structure, 26(2), 209-224.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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