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Mouse Anti-ANKS4B Recombinant Antibody (3E3) (CBMAB-A0372-LY)

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Published Data
Mouse Anti-ANKS4B Recombinant Antibody (3E3) in IF
Mouse Anti-ANKS4B Recombinant Antibody (3E3) in IF
The BHB motif of ANKS4B is essential for ANKS4B LK segment targeting. A, diagram of the constructs used to interrogate the apical targeting contribution of motifs found in the ANKS4B LK segment through chimeric and point mutation (arrow) analysis. B-E, confocal images of 12-day polarized CACO-2BBE cells stably expressing EGFP-fusion constructs tested. The BHB motif plays an essential role in apical targeting. The boxed regions denote areas in the zoomed panels for each figure. The dashed lines indicate the positions where the x-z sections were taken; the x-z sections are shown below each en face image. Scale bars, 10 μm. F and G, scatter plot quantification of the BB:cytosol ratios of EGFP signal for all EGFP-fusion constructs tested. The data are derived from three independent immunostaining experiments. At least 50 BB:cytosol ratio data points were collected from cells for each plot variable. ***, p < 0.0001, t test. The bars indicate means ± S.D. ...View More
Graves, M. J., Matoo, S., Choi, M. S., Storad, Z. A., Idris, R. A. E. S., Pickles, B. K., ... & Crawley, S. W. (2020). A cryptic sequence targets the adhesion complex scaffold ANKS4B to apical microvilli to promote enterocyte brush border assembly. Journal of Biological Chemistry, 295(36), 12588-12604.
Mouse Anti-ANKS4B Recombinant Antibody (3E3) in Immunoblot
Mouse Anti-ANKS4B Recombinant Antibody (3E3) in Immunoblot
Immunoblot analysis of a CACO-2BBE differentiation time series for expression of ANKS4B and CALML4. GAPDH served as a loading control. ANKS4B is detected across the entire time series as multiple bands, with the lowest-mass species (denote by an arrow) exhibiting a marked increase in total cellular levels during polarization. ...View More
Graves, M. J., Matoo, S., Choi, M. S., Storad, Z. A., Idris, R. A. E. S., Pickles, B. K., ... & Crawley, S. W. (2020). A cryptic sequence targets the adhesion complex scaffold ANKS4B to apical microvilli to promote enterocyte brush border assembly. Journal of Biological Chemistry, 295(36), 12588-12604.
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Mouse
Clone
3E3
Application
WB, ELISA
Immunogen
ANKS4B (NP_665872.1, 1 a.a. ~ 417 a.a) full-length recombinant protein with GST tag.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
ankyrin repeat and sterile alpha motif domain containing 4B
Entrez Gene ID
257629
UniProt ID
Q8N8V4
Alternative Names
FLJ38819; HARP; MGC133380; MGC133381
Function
As part of the intermicrovillar adhesion complex/IMAC plays a role in epithelial brush border differentiation, controlling microvilli organization and length. Plays a role in assembly of the complex (PubMed:26812018). May play a role in cellular response to endoplasmic reticulum stress (By similarity).
Biological Process
Brush border assembly Source: UniProtKB
Cell differentiation Source: UniProtKB-KW
Cellular protein-containing complex assembly Source: UniProtKB
Protein localization to microvillus Source: UniProtKB
Response to endoplasmic reticulum stress Source: UniProtKB
Cellular Location
Microvillus. Localizes at the tip of microvilli (PubMed:26812018). May associate with endoplasmic reticulum membranes (By similarity).

Graves, M. J., Matoo, S., Choi, M. S., Storad, Z. A., Idris, R. A. E. S., Pickles, B. K., ... & Crawley, S. W. (2020). A cryptic sequence targets the adhesion complex scaffold ANKS4B to apical microvilli to promote enterocyte brush border assembly. Journal of Biological Chemistry, 295(36), 12588-12604.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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