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Mouse Anti-ANKZF1 Antibody (B12) (CBMAB-0124-YC)

Provided herein are mouse monoclonal antibodies against Human ANKZF1. The antibody clone 1A12 can be used for immunoassay techniques, such as IP, WB and MA.
See all ANKZF1 antibodies
Published Data
Mouse Anti-ANKZF1 Antibody (B12) in IF
Immunostaining showing effects of ANKZF1 RNAi or OE on C-I30-FLAG aggregation. ...View More
Wu, Z., Tantray, I., Lim, J., Chen, S., Li, Y., Davis, Z., ... & Lu, B. (2019). MISTERMINATE mechanistically links mitochondrial dysfunction with proteostasis failure. Molecular cell, 75(4), 835-848.
Mouse Anti-ANKZF1 Antibody (B12) in IP
CoIP assay showing effect of Parkin on the interaction of ANKZF1 or NEMF with ABCE1. ...View More
Wu, Z., Tantray, I., Lim, J., Chen, S., Li, Y., Davis, Z., ... & Lu, B. (2019). MISTERMINATE mechanistically links mitochondrial dysfunction with proteostasis failure. Molecular cell, 75(4), 835-848.

Summary

Host Animal
Mouse
Specificity
Human
Clone
B12
Antibody Isotype
IgG2b, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 68-355 mapping within an internal region of ANKZF1 of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2b, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ANKZF1
Introduction
Ankyrin repeat and zinc finger domain-containing protein 1 (ANKZF1) is a protein that in humans is encoded by the ANKZF1 gene. ANKZF1 contains two ANK repeats and one C2H2-type zinc finger.
Entrez Gene ID
55139
UniProt ID
Q9H8Y5
Alternative Names
FLJ10415; ZNF744
Function
Plays a role in the cellular response to hydrogen peroxide and in the maintenance of mitochondrial integrity under conditions of cellular stress (PubMed:28302725). Involved in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway (By similarity).
Biological Process
Cellular response to hydrogen peroxide Source: UniProtKB
Ubiquitin-dependent ERAD pathway Source: GO_Central
Cellular Location
Cytoplasm. Translocates to the mitochondria upon exposure to hydrogen peroxide.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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