Mouse Anti-ANP32B Recombinant Antibody (8E10) (CBMAB-A2835-YC)

Name: Mouse Anti-ANP32B Recombinant Antibody (8E10)
SKU: CBMAB-A2835-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

8E10

Application

ELISA, IF, WB

Immunogen

ANP32B (NP_006392. 1, 1-251 aa) full-length recombinant protein with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF(ICC)	10 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 1-251

More Infomation

Target

Full Name

Acidic Nuclear Phosphoprotein 32 Family Member B

Introduction

Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) in

Entrez Gene ID

10541

UniProt ID

Q92688

Alternative Names

Acidic Nuclear Phosphoprotein 32 Family Member B; Acidic Protein Rich In Leucines; Acidic (Leucine-Rich) Nuclear Phosphoprotein 32 Family, Member B; Putative HLA-DR-Associated Protein I-2; Silver-Stainable Protein SSP29; PHAPI2; APRIL; Acidic Leucine-Rich

Function

Multifunctional protein that is involved in the regulation of many processes including cell proliferation, apoptosis, cell cycle progression or transcription (PubMed:20015864, PubMed:18039846). Regulates the proliferation of neuronal stem cells, differentiation of leukemic cells and progression from G1 to S phase of the cell cycle. As negative regulator of caspase-3-dependent apoptosis, may act as an antagonist of ANP32A in regulating tissue homeostasis (PubMed:20015864). Exhibits histone chaperone properties, able to recruit histones to certain promoters, thus regulating the transcription of specific genes (PubMed:20538007, PubMed:18039846). Plays also an essential role in the nucleocytoplasmic transport of specific mRNAs via the uncommon nuclear mRNA export receptor XPO1/CRM1 (PubMed:17178712). Participates in the regulation of adequate adaptive immune responses by acting on mRNA expression and cell proliferation (By similarity).
(Microbial infection) Plays an essential role in influenza A and B viral genome replication (PubMed:33045004, PubMed:31217244). Plays also a role in foamy virus mRNA export from the nucleus to the cytoplasm (PubMed:21159877).

Biological Process

Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
Inner ear development Source: Ensembl
Negative regulation of cell differentiation Source: UniProtKB
Nucleosome assembly Source: UniProtKB
Positive regulation of protein export from nucleus Source: UniProtKB
Regulation of apoptotic process Source: GO_Central
Roof of mouth development Source: Ensembl
Vasculature development Source: Ensembl
Ventricular system development Source: Ensembl

Cellular Location

Isoform 1: Cytoplasm; Nucleus. Accumulates in the nuclei at the S phase.
Isoform 2: Cytoplasm. Lacks a nuclear localization signal.

PTM

Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity).
Directly cleaved by caspase-3/CASP3.

Rivero-Rodríguez, F., Díaz-Quintana, A., Velázquez-Cruz, A., González-Arzola, K., Gavilan, M. P., Velázquez-Campoy, A., ... & Díaz-Moreno, I. (2021). Inhibition of the PP2A activity by the histone chaperone ANP32B is long-range allosterically regulated by respiratory cytochrome c. Redox biology, 43, 101967.

Yang, S., Zhu, X. N., Zhang, H. L., Yang, Q., Wei, Y. S., Zhu, D., ... & Yu, Y. (2021). ANP32B-mediated repression of p53 contributes to maintenance of normal and CML stem cells. Blood.

Günther, M., Bauer, A., Müller, M., Zaeck, L., & Finke, S. (2020). Interaction of host cellular factor ANP32B with matrix proteins of different paramyxoviruses. Journal of General Virology, 101(1), 44-58.

Park, Y. H., Chungu, K., Lee, S. B., Woo, S. J., Cho, H. Y., Lee, H. J., ... & Han, J. Y. (2020). Host-specific restriction of avian influenza virus caused by differential dynamics of ANP32 family members. The Journal of infectious diseases, 221(1), 71-80.

Chemnitz, J., Pieper, D., Stich, L., Schumacher, U., Balabanov, S., Spohn, M., ... & Zinser, E. (2019). The acidic protein rich in leucines Anp32b is an immunomodulator of inflammation in mice. Scientific reports, 9(1), 1-14.

Barbeito, P., Sarandeses, C. S., Díaz-Jullien, C., Muras, J., Covelo, G., Moreira, D., ... & Freire, M. (2017). Prothymosin α interacts with SET, ANP32A and ANP32B and other cytoplasmic and mitochondrial proteins in proliferating cells. Archives of biochemistry and biophysics, 635, 74-86.

Ohno, Y., Koizumi, M., Nakayama, H., Watanabe, T., Hirooka, M., Tokumoto, Y., ... & Hiasa, Y. (2017). Downregulation of ANP32B exerts anti-apoptotic effects in hepatocellular carcinoma. PloS one, 12(5), e0177343.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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