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Mouse Anti-ANP32E Recombinant Antibody (CBYJL-076) (CBMAB-L0131-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to Acidic Nuclear Phosphoprotein 32 Family Member E (ANP32E). The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all ANP32E antibodies
Published Data
Mouse Anti-ANP32E Recombinant Antibody (CBYJL-076) in WB
FISCs were cultured for 12 h and transfected with siNC or siLockd oligos for 48 h. ANP32E protein was detected by western blot using α-tubulin as the internal loading control. ...View More
Chen, X., Xue, G., Zhao, J., Zhang, Y., Zhang, S., Wang, W., ... & Wang, H. (2022). Lockd promotes myoblast proliferation and muscle regeneration via binding with DHX36 to facilitate 5' UTR rG4 unwinding and Anp32e translation. Cell Reports, 39(10).
Mouse Anti-ANP32E Recombinant Antibody (CBYJL-076) in IF
EdU labeling was performed in the ASCs transfected with siNC or siAnp32e oligos for 6 h at 48 h post-transfection. ...View More
Chen, X., Xue, G., Zhao, J., Zhang, Y., Zhang, S., Wang, W., ... & Wang, H. (2022). Lockd promotes myoblast proliferation and muscle regeneration via binding with DHX36 to facilitate 5' UTR rG4 unwinding and Anp32e translation. Cell Reports, 39(10).

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYJL-076
Antibody Isotype
IgM, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 46-68 near the N-terminus of LANPL of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgM, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Acidic Nuclear Phosphoprotein 32 Family Member E
Introduction
Histone chaperone that specifically mediates the genome-wide removal of histone H2A.Z/H2AFZ from the nucleosome: removes H2A.Z/H2AFZ from its normal sites of deposition, especially from enhancer and insulator regions. ANP32E isn't involved in deposition of H2A.Z/H2AFZ in the nucleosome. ANP32E may stabilize the evicted H2A.Z/H2AFZ-H2B dimer, thus shifting the equilibrium towards dissociation and the off-chromatin state (PubMed:24463511). ANP32E inhibits activity of protein phosphatase 2A (PP2A). ANP32E does not inhibit protein phosphatase 1. ANP32E may play a role in cerebellar development and synaptogenesis.
Entrez Gene ID
81611
UniProt ID
Q9BTT0
Alternative Names
Acidic Nuclear Phosphoprotein 32 Family Member E; Acidic (Leucine-Rich) Nuclear Phosphoprotein 32 Family; Member E; LANP-Like Protein; LANP-L; Acidic Leucine-Rich Nuclear Phosphoprotein 32 Family Member E; Leucine-Rich Acidic Nuclear Protein Like; LANPL
Function
Histone chaperone that specifically mediates the genome-wide removal of histone H2A.Z/H2AZ1 from the nucleosome: removes H2A.Z/H2AZ1 from its normal sites of deposition, especially from enhancer and insulator regions. Not involved in deposition of H2A.Z/H2AZ1 in the nucleosome. May stabilize the evicted H2A.Z/H2AZ1-H2B dimer, thus shifting the equilibrium towards dissociation and the off-chromatin state (PubMed:24463511). Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis.
Biological Process
Histone exchange Source: UniProtKB
Regulation of apoptotic process Source: GO_Central
Cellular Location
Nucleus; Cytoplasm
PTM
Phosphorylated. The phosphorylation is nuclear localization signal (NLS)-dependent (By similarity).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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