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Mouse Anti-ANXA11 Recombinant Antibody (D-12) (CBMAB-A2892-YC)

Provided herein is a Mouse monoclonal antibody against Human Annexin A11. The antibody can be used for immunoassay techniques, such as WB, IP, IF, IHC-P, ELISA.
See all ANXA11 antibodies
Published Data
Mouse Anti-ANXA11 Recombinant Antibody (D-12) in IHC
HSF1 and ANXA11 expression levels as detected in the normal and tumor tissues by IHC (immunohistochemistry) (scale bar, 50 µm and 100 µm). ...View More
Shen, Q., Wang, R., Liu, X., Song, P., Zheng, M., Ren, X., ... & Li, J. (2022). HSF1 stimulates glutamine transport by super-enhancer-driven lncRNA LINC00857 in colorectal cancer. Cancers, 14(16), 3855.
Mouse Anti-ANXA11 Recombinant Antibody (D-12) in WB
The effect of ANXA11 knockdown on SLC1A5 expression in CRC cells, as explored by Western blotting. ...View More
Shen, Q., Wang, R., Liu, X., Song, P., Zheng, M., Ren, X., ... & Li, J. (2022). HSF1 stimulates glutamine transport by super-enhancer-driven lncRNA LINC00857 in colorectal cancer. Cancers, 14(16), 3855.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
D-12
Antibody Isotype
IgG2a, κ
Application
WB, IP, IF, IHC-P, ELISA

Basic Information

Immunogen
Amino acids 376-420 of Annexin XI of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Annexin A11
Introduction
ANXA11 is a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and conserved C-terminal domains, which contain calcium-dependent phospholipid-binding sites. The encoded protein
Entrez Gene ID
Human311
Mouse11744
Rat290527
UniProt ID
HumanP50995
MouseP97384
RatQ5XI77
Alternative Names
Annexin A11; Calcyclin-Associated Annexin 50; 56 KDa Autoantigen; Annexin XI; Annexin-11; CAP-50; ANX11; CAP50;
Function
Binds specifically to calcyclin in a calcium-dependent manner (By similarity). Required for midbody formation and completion of the terminal phase of cytokinesis.
Biological Process
Cytokinetic process Source: UniProtKB
Phagocytosis Source: UniProtKB
Response to calcium ion Source: UniProtKB
Cellular Location
Spindle; Nucleus envelope; Nucleoplasm; Cytoplasm; Melanosome. Found throughout the nucleoplasm at interphase and during mitosis concentrates around the mitotic apparatus (By similarity). Elevation of intracellular calcium causes relocalization from the nucleoplasm to the nuclear envelope, with little effect on the cytoplasmic pool. Localization to the nuclear envelope is cell-cycle dependent.
Involvement in disease
Amyotrophic lateral sclerosis 23 (ALS23): A form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. The pathologic hallmarks of the disease include pallor of the corticospinal tract due to loss of motor neurons, presence of ubiquitin-positive inclusions within surviving motor neurons, and deposition of pathologic aggregates. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases. ALS23 is an autosomal dominant form with incomplete penetrance.

Oblak, I. (2021). Agregacijske študije proteina aneksin A11 ter njegovih mutantov D40G in Δ119.

Sainouchi, M., Hatano, Y., Tada, M., Ishihara, T., Ando, S., Kato, T., ... & Kakita, A. (2021). A novel splicing variant of ANXA11 in a patient with amyotrophic lateral sclerosis: histologic and biochemical features. Acta neuropathologica communications, 9(1), 1-9.

Lillebostad, P. A., Raasakka, A., Hjellbrekke, S. J., Patil, S., Røstbø, T., Hollås, H., ... & Kursula, P. (2020). Structure of the ALS Mutation Target Annexin A11 Reveals a Stabilising N-Terminal Segment. Biomolecules, 10(4), 660.

Liao, Y. C., Fernandopulle, M. S., Wang, G., Choi, H., Hao, L., Drerup, C. M., ... & Ward, M. E. (2019). RNA granules hitchhike on lysosomes for long-distance transport, using annexin A11 as a molecular tether. Cell, 179(1), 147-164.

Maklin, A. (2019). Priprava rekombinantnega aneksina A11 v bakterijah E. coli z uporabo ekspresijskih vektorjev na osnovi pMCSG7 (Doctoral dissertation, Univerza v Ljubljani, Fakulteta za kemijo in kemijsko tehnologijo).

Hua, K., Li, Y., Zhao, Q., Fan, L., Tan, B., & Gu, J. (2018). Downregulation of annexin A11 (ANXA11) inhibits cell proliferation, invasion, and migration via the AKT/GSK-3β pathway in gastric cancer. Medical science monitor: international medical journal of experimental and clinical research, 24, 149.

Liao, D., Liao, Q., Huang, C., & Bi, F. (2018). Mutations of G38R and D40G cause amyotrophic lateral sclerosis by reducing Annexin A11 protein stability. Zhong nan da xue xue bao. Yi xue ban= Journal of Central South University. Medical sciences, 43(6), 577-582.

Smith, B. N., Topp, S. D., Fallini, C., Shibata, H., Chen, H. J., Troakes, C., ... & Shaw, C. E. (2017). Mutations in the vesicular trafficking protein annexin A11 are associated with amyotrophic lateral sclerosis. Science translational medicine, 9(388).

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For research use only. Not intended for any clinical use.

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