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Mouse Anti-ANXA7 Recombinant Antibody (A-1) (CBMAB-A2941-YC)

Provided herein is a Mouse monoclonal antibody against Human Annexin A7. The antibody can be used for immunoassay techniques, such as WB, IP, IF, IHC-P, ELISA.
See all ANXA7 antibodies
Published Data
Mouse Anti-ANXA7 Recombinant Antibody (A-1) in IF
Immunofluorescence (IF) assay of the co-localization analysis of ANXA7 and Hsc70. (a-b), A549 cells were treated with ZBM-H (5 μM) for 24 h, and the co-localization of ANXA7 and Hsc70 was analyzed and visualized by confocal immunofluorescence microscopy. Scale bar: 20 μm, n = 3. ...View More
Ning, J., Cui, X., Li, N., Li, N., Zhao, B., Miao, J., & Lin, Z. (2022). Activation of GRP78 ATPase suppresses A549 lung cancer cell migration by promoting ITGB4 degradation. Cell Adhesion & Migration, 16(1), 107-114.
Mouse Anti-ANXA7 Recombinant Antibody (A-1) in IP
Co-immunoprecipitation (Co-IP) of ANXA7 with XRN2 from HUVECs treated with 25 and 50 μm ABO for 6 h. Bar chart shows quantification of the protein levels of ANXA7. ...View More
He, X., Zhao, X., Su, L., Zhao, B., & Miao, J. (2019). MROH 7-TTC 4 read-through lnc RNA suppresses vascular endothelial cell apoptosis and is upregulated by inhibition of ANXA 7 GTP ase activity. The FEBS Journal, 286(24), 4937-4950.
Mouse Anti-ANXA7 Recombinant Antibody (A-1) in IP
Western blot analysis of co-immunoprecipitation of ANXA7 and its phosphorylation in HEK293T RKIP−/− cells transfected with expression vectors pCMV6 and pCMV6-RKIP for 24 h, then treated with 20 µM SEC for 3 h. ANXA7 and its phosphorylation level were quantified. ...View More
Liu, S., Li, X., Lin, Z., Su, L., Yan, S., Zhao, B., & Miao, J. (2018). SEC-induced activation of ANXA7 GTPase suppresses prostate cancer metastasis. Cancer letters, 416, 11-23.
Mouse Anti-ANXA7 Recombinant Antibody (A-1) in WB
Western blot analysis of urinary EVs collected from six healthy volunteers and six bladder cancer patients (stage pT1-pT3). HEXB bands are predicted at two distinct protein sizes. EV marker Alix was used as a loading control. ...View More
Silvers, C. R., Miyamoto, H., Messing, E. M., Netto, G. J., & Lee, Y. F. (2017). Characterization of urinary extracellular vesicle proteins in muscle-invasive bladder cancer. Oncotarget, 8(53), 91199.
Mouse Anti-ANXA7 Recombinant Antibody (A-1) in WB
Effects of the amyloid peptide on the relative abundance of selected plasma membrane proteins in microglia cells. Western blot analyses of Neuropilin 1 (NRP1), Annexin VII (ANXA5), Annexin V (ANXA7), Interferon-induced transmembrane protein 3, (IFTM3) MAP/microtubule affinity-regulating kinase 3 (MARK3) of the microsomal fraction of untreated (-) and Aβ1-42 (+) treated primary microglia are reported. ...View More
Correani, V., Di Francesco, L., Mignogna, G., Fabrizi, C., Leone, S., Giorgi, A., ... & Schininà, M. E. (2017). Plasma Membrane Protein Profiling in Beta-Amyloid-Treated Microglia Cell Line. Proteomics, 17(17-18), 1600439.
Mouse Anti-ANXA7 Recombinant Antibody (A-1) in IP and IF
Interaction of RACK1 with Annexin A7. (A) RACK1 was found to co-immunoprecipitate with Annexin A7 in control cells, but not the RACK1 down-regulated cells. (B) Co-localization of RACK1 and Annexin A7 is shown in the cytoplasma by yellow cellular staining. Scale bars = 25lm ...View More
Du, Y., Meng, J., Huang, Y., Wu, J., Wang, B., Ibrahim, M. M., & Tang, J. (2014). Guanine nucleotide-binding protein subunit beta-2-like 1, a new Annexin A7 interacting protein. Biochemical and Biophysical Research Communications, 445(1), 58-63.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
A-1
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, IHC-P, ELISA

Basic Information

Immunogen
Amino acids 9-119 mapping near the C-terminus of Annexin VII of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Annexin A7
Introduction
Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and
Entrez Gene ID
Human310
Mouse11750
Rat155423
UniProt ID
HumanP20073
MouseQ07076
RatQ6IRJ7
Alternative Names
Annexin A7; Annexin VII; Annexin-7; SYNEXIN; ANX7; SNX;
Function
Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.
Biological Process
Autophagy Source: UniProtKB
Epithelial cell differentiation Source: UniProtKB
Membrane fusion Source: Ensembl
Negative regulation of gene expression Source: UniProtKB
Response to calcium ion Source: Ensembl
Response to organic cyclic compound Source: Ensembl
Cellular Location
Endoplasmic reticulum membrane; Extracellular exosome; Nucleus; Collagen-containing extracellular matrix; Cytoplasm; Membrane

Alauddin, M., Salker, M. S., Umbach, A. T., Rajaxavier, J., Okumura, T., Singh, Y., ... & Lang, F. (2020). Annexin A7 regulates endometrial receptivity. Frontiers in cell and developmental biology, 8, 770.

He, X., Lin, Z., Ning, J., Li, N., Cui, X., Zhao, B., ... & Miao, J. (2020). Promoting TTC4 and HSP70 interaction and translocation of annexin A7 to lysosome inhibits apoptosis in vascular endothelial cells. The FASEB Journal, 34(9), 12932-12945.

Deng, Q., Li, L., & Jin, Y. (2020). Long Noncoding RNAs Coregulated by Annexin A7 and JNK in Hepatocellular Carcinoma Cells Identified by Whole-Genome Expression Profiling. BioMed Research International, 2020.

Gui, S. J., Ding, R. L., Wan, Y. P., Zhou, L., Chen, X. J., Zeng, G. Q., & He, C. Z. (2020). Knockdown of annexin VII enhances nasopharyngeal carcinoma cell radiosensitivity in vivo and in vitro. Cancer Biomarkers, 28(2), 129-139.

Lin, Q. S., Wang, W. X., Lin, Y. X., Lin, Z. Y., Yu, L. H., Kang, Y., & Kang, D. Z. (2019). Annexin A7 induction of neuronal apoptosis via effect on glutamate release in a rat model of subarachnoid hemorrhage. Journal of neurosurgery, 132(3), 777-787.

Borst, O., Geue, S., Manke, M. C., Peng, B., Muenzer, P., Kollotzek, F., ... & Gawaz, M. (2020). Annexin A7 is a critical regulator of Ca2+ mobilization and lipid metabolism during platelet activation and arterial thrombosis. European Heart Journal, 41(Supplement_2), ehaa946-3774.

Huang, Y., Wang, H., & Yang, Y. (2018). Annexin A7 is correlated with better clinical outcomes of patients with breast cancer. Journal of cellular biochemistry, 119(9), 7577-7584.

Li, H., Liu, S., Sun, X., Yang, J., Yang, Z., Shen, H., ... & Chen, G. (2018). Critical role for Annexin A7 in secondary brain injury mediated by its phosphorylation after experimental intracerebral hemorrhage in rats. Neurobiology of disease, 110, 82-92.

Leighton, X., Bera, A., Eidelman, O., Bubendorf, L., Zellweger, T., Banerjee, J., ... & Srivastava, M. (2018). Tissue microarray analysis delineate potential prognostic role of Annexin A7 in prostate cancer progression. Plos one, 13(10), e0205837.

Gao, F., Li, D., Rui, Q., Ni, H., Liu, H., Jiang, F., ... & Dang, B. (2018). Annexin A7 levels increase in rats with traumatic brain injury and promote secondary brain injury. Frontiers in neuroscience, 12, 357.

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For research use only. Not intended for any clinical use.

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