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Mouse Anti-AP2B1 Recombinant Antibody (2D5) (CBMAB-A2971-YC)

Provided herein is a Mouse monoclonal antibody against Human Adaptor Related Protein Complex 2 Beta 1 Subunit. The antibody can be used for immunoassay techniques, such as ELISA, IHC-P, WB.
See all AP2B1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2D5
Antibody Isotype
IgG1, κ
Application
ELISA, IHC-P, WB

Basic Information

Immunogen
AP2B1 (NP_001273.1, 585 a.a. ~ 651 a.a) partial recombinant protein with GST tag.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IHC-P3 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
adaptor-related protein complex 2, beta 1 subunit
Introduction
AP2B1 is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane.
Entrez Gene ID
163
UniProt ID
P63010
Alternative Names
Adaptor Related Protein Complex 2 Beta 1 Subunit; Clathrin Assembly Protein Complex 2 Beta Large Chain; Plasma Membrane Adaptor HA2/AP2 Adaptin Beta Subunit; Adaptor-Related Protein Complex 2 Subunit Beta; Adaptor Protein Complex AP-2 Subunit Beta; Beta-2
Function
Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10 (PubMed:23676497). The AP-2 beta subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins; at least some clathrin-associated sorting proteins (CLASPs) are recognized by their [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif. The AP-2 beta subunit binds to clathrin heavy chain, promoting clathrin lattice assembly; clathrin displaces at least some CLASPs from AP2B1 which probably then can be positioned for further coat assembly.
Biological Process
Antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
Aorta development Source: Ensembl
Clathrin coat assembly Source: Ensembl
Clathrin-dependent endocytosis Source: UniProtKB
Coronary vasculature development Source: Ensembl
Ephrin receptor signaling pathway Source: Reactome
Intracellular protein transport Source: InterPro
Kidney development Source: Ensembl
Low-density lipoprotein particle clearance Source: Reactome
Low-density lipoprotein particle receptor catabolic process Source: Reactome
Membrane organization Source: Reactome
Negative regulation of neuron death Source: Ensembl
Positive regulation of endocytosis Source: Ensembl
Positive regulation of protein localization to membrane Source: Ensembl
Postsynaptic neurotransmitter receptor internalization Source: Ensembl
Regulation of defense response to virus by virus Source: Reactome
Synaptic vesicle endocytosis Source: SynGO
Ventricular septum development Source: Ensembl
Vesicle-mediated transport Source: GO_Central
Wnt signaling pathway, planar cell polarity pathway Source: Reactome
Cellular Location
Cell membrane; Coated pit. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.
PTM
Phosphorylation at Tyr-737 by SRC occurs at the plasma membrane in clathrin-coated vesicles (CCVs).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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