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Mouse Anti-AP5S1 Recombinant Antibody (E-4) (CBMAB-A3001-YC)

Provided herein is a Mouse monoclonal antibody against Human Adaptor Related Protein Complex 5 Sigma 1 Subunit. The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all AP5S1 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
E-4
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 32-53 within an internal region of AP5S1 of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Adaptor Related Protein Complex 5 Sigma 1 Subunit
Introduction
As part of AP-5, a probable fifth adaptor protein complex it may be involved in endosomal transport. According to PubMed:20613862, it is required for efficient homologous recombination DNA double-strand break repair.
Entrez Gene ID
Human55317
Mouse69596
Rat499893
UniProt ID
HumanQ9NUS5
MouseQ9D742
RatD3ZGQ9
Alternative Names
Adaptor Related Protein Complex 5 Sigma 1 Subunit; Adaptor-Related Protein Complex 5 Sigma Subunit; C20orf29; Sigma5; Adaptor-Related Protein Complex 5, Sigma 1 Subunit; Adapter-Related Protein Complex 5 Sigma Subunit; Chromosome 20 Open Reading Frame 29;
Function
As part of AP-5, a probable fifth adaptor protein complex it may be involved in endosomal transport. According to PubMed:20613862, it is required for efficient homologous recombination DNA double-strand break repair.
Biological Process
Double-strand break repair via homologous recombination Source: UniProtKB
Endosomal transport Source: UniProtKB
Protein transport Source: UniProtKB-KW
Cellular Location
Late endosome membrane; Cytosol; Lysosome membrane
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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