Mouse Anti-APBB1 Recombinant Antibody (D11) (CBMAB-A3016-YC)

Name: Mouse Anti-APBB1 Recombinant Antibody (D11)
SKU: CBMAB-A3016-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-APBB1 Recombinant Antibody (D11) in IF

Immunofluorescence for MAP1B (white), Fe65 (red), APP (green), and DAPI for nuclei staining (blue) in mice hippocampal cells in control and treatment with Aβo (0.5 μM, 24 h). ...View More

Godoy, P. A., Mennickent, D., Cuchillo-Ibanez, I., Ramírez-Molina, O., Silva-Grecchi, T., Panes-Fernández, J., ... & Fuentealba, J. (2021). Increased P2× 2 receptors induced by amyloid-β peptide participates in the neurotoxicity in alzheimer's disease. Biomedicine & Pharmacotherapy, 142, 111968.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

D11

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 63-101 near the N-terminus of Fe65 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG3, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 254-293

More Infomation

Target

Full Name

Amyloid Beta Precursor Protein Binding Family B Member 1

Introduction

APBB1 is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related

Entrez Gene ID

11785

UniProt ID

Q9QXJ1

Alternative Names

Amyloid Beta Precursor Protein Binding Family B Member 1; Amyloid Beta (A4) Precursor Protein-Binding, Family B, Member 1 (Fe65); FE65; RIR; Amyloid Beta A4 Precursor Protein-Binding Family B Member 1; Amyloid-Beta A4 Precursor Protein-Binding Family B Me

Function

Transcription coregulator that can have both coactivator and corepressor functions. Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its transcription activation activity. Functions in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s).

Biological Process

Apoptotic process Source: UniProtKB-KW
Axonogenesis Source: UniProtKB
Cell cycle arrest Source: UniProtKB
Cellular response to DNA damage stimulus Source: UniProtKB
Double-strand break repair Source: Reactome
Histone H4 acetylation Source: UniProtKB
Negative regulation of cell growth Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: ARUK-UCL
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of protein secretion Source: ARUK-UCL
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: ARUK-UCL
Regulation of transcription, DNA-templated Source: GO_Central
Signal transduction Source: UniProtKB

Cellular Location

Cytoplasm; Cell membrane; Nucleus; Nucleus speckle; Growth cone. Colocalizes with TSHZ3 in axonal growth cone (By similarity). In normal conditions, it mainly localizes to the cytoplasm, while a small fraction is tethered to the cell membrane via its interaction with APP. Following exposure to DNA damaging agents, it is released from cell membrane and translocates to the nucleus. Nuclear translocation is under the regulation of APP. Colocalizes with TSHZ3 in the nucleus. Colocalizes with NEK6 at the nuclear speckles. Phosphorylation at Ser-610 by SGK1 promotes its localization to the nucleus (By similarity).

PTM

Polyubiquitination by RNF157 leads to degradation by the proteasome (PubMed:25342469).
Phosphorylation at Ser-610 by SGK1 promotes its localization to the nucleus (By similarity). Phosphorylated following nuclear translocation. Phosphorylation at Tyr-547 by ABL1 enhances transcriptional activation activity and reduces the affinity for RASD1/DEXRAS1.

Lee, J. H., Kim, J. Y., Kim, S. Y., Im Choi, S., Kim, K. C., Cho, E. W., & Kim, I. G. (2017). APBB1 reinforces cancer stem cell and epithelial-to-mesenchymal transition by regulating the IGF1R signaling pathway in non-small-cell lung cancer cells. Biochemical and biophysical research communications, 482(1), 35-42.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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