Mouse Anti-APC2 Recombinant Antibody (CBT4818) (V2LY-0625-LY894)

Name: Mouse Anti-APC2 Recombinant Antibody (CBT4818)
SKU: V2LY-0625-LY894
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-APC2 Recombinant Antibody (CBT4818) (V2LY-0625-LY894) in ELISA

Mouse Anti-APC2 Recombinant Antibody (CBT4818) (V2LY-0625-LY894) in WB

Mouse Anti-APC2 Recombinant Antibody (CBT4818) (V2LY-0625-LY894) in IF

Mouse Anti-APC2 Recombinant Antibody (CBT4818) (V2LY-0625-LY894) in FC

Mouse Anti-APC2 Recombinant Antibody (CBT4818) (V2LY-0625-LY894) in IHC

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT4818

Application

IHC, ICC, FC

Immunogen

Purified recombinant fragment of human APC2 (AA: 2041-2181) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC-P	1:200-1:1,000
ICC	1:200-1:1,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

APC regulator of WNT signaling pathway 2

Entrez Gene ID

10297

UniProt ID

O95996

Function

Stabilizes microtubules and may regulate actin fiber dynamics through the activation of Rho family GTPases (PubMed:25753423).
May also function in Wnt signaling by promoting the rapid degradation of CTNNB1 (PubMed:10021369, PubMed:11691822, PubMed:9823329).

Biological Process

Activation of GTPase activity
Cell fate specification
Cell migration
Microtubule cytoskeleton organization
Negative regulation of canonical Wnt signaling pathway
Negative regulation of microtubule depolymerization
Nervous system development
Pattern specification process
Proteasome-mediated ubiquitin-dependent protein catabolic process
Wnt signaling pathway

Cellular Location

Cytoplasm, cytoskeleton
Golgi apparatus
Cytoplasm
Cytoplasm, perinuclear region

Involvement in disease

Intellectual developmental disorder, autosomal recessive 74 (MRT74):
A disorder characterized by intellectual impairment, macrocephaly, and dysmorphic features. Epilepsy with eyelid myoclonus has also been reported.
Cortical dysplasia, complex, with other brain malformations 10 (CDCBM10):
An autosomal recessive disorder of aberrant neuronal migration during brain development. CDCBM10 is clinically characterized by onset in infancy of global developmental delay, impaired intellectual development, seizures, inability to ambulate, and absent language. Brain imaging shows lissencephaly, cortical dysplasia, subcortical heterotopia, and paucity of white matter.

Meng, F., Liu, X., Lin, C., Xu, L., Liu, J., Zhang, P., ... & Zhang, Y. (2020). SMYD2 suppresses APC2 expression to activate the Wnt/β-catenin pathway and promotes epithelial-mesenchymal transition in colorectal cancer. American journal of cancer research, 10(3), 997.

Mohamed, N. E., Hay, T., Reed, K. R., Smalley, M. J., & Clarke, A. R. (2019). APC2 is critical for ovarian WNT signalling control, fertility and tumour suppression. BMC cancer, 19(1), 1-16.

Geng, Y., Zheng, X., Hu, W., Wang, Q., Xu, Y., He, W., ... & Jiang, J. (2019). Hsa_circ_0009361 acts as the sponge of miR-582 to suppress colorectal cancer progression by regulating APC2 expression. Clinical Science, 133(10), 1197-1213.

Zhang, K., Wang, J., Yang, L., Yuan, Y. C., Tong, T. R., Wu, J., ... & Raz, D. J. (2018). Targeting histone methyltransferase G9a inhibits growth and Wnt signaling pathway by epigenetically regulating HP1α and APC2 gene expression in non-small cell lung cancer. Molecular cancer, 17(1), 1-15.

Young, M. A., Daly, C. S., Taylor, E., James, R., Clarke, A. R., & Reed, K. R. (2018). Subtle Deregulation of the Wnt‐Signaling Pathway Through Loss of Apc2 Reduces the Fitness of Intestinal Stem Cells. Stem Cells, 36(1), 114-122.

Wu, Z., Shi, W., & Jiang, C. (2018). Overexpressing circular RNA hsa_circ_0002052 impairs osteosarcoma progression via inhibiting Wnt/β-catenin pathway by regulating miR-1205/APC2 axis. Biochemical and biophysical research communications, 502(4), 465-471.

Dokanehiifard, S., & Soltani, B. M. (2018). Hsa-miR-11181 regulates Wnt signaling pathway through targeting of APC2 transcripts in SW480 cell line. Gene, 641, 297-302.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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