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Please try the standard protocols which include: protocols, troubleshooting and guide.
Enzyme-linked Immunosorbent Assay (ELISA)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Fig.1 The pure Stx2, HuSAP, and the immune complex eluted from the affinity column were run on the same SDS-PAGE, and immunoblotted with antibodies against Stx2, HuSAP, or a mixture of antibodies against Stx2 and HuSAP, respectively.
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