Rabbit Anti-APOBEC3G Recombinant Antibody (CBYY-C1865) (CBMAB-C3303-YY)

Name: Rabbit Anti-APOBEC3G Recombinant Antibody (CBYY-C1865)
SKU: CBMAB-C3303-YY
Rating: 5 (1 reviews)

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Published Data

Rabbit Anti-APOBEC3G Recombinant Antibody (CBYY-C1865) in WB

Western blot analysis with lysates of control or IRGM+/− HT-29 cells or DYKDDDDK IRGM complemented IRGM+/− HT-29 cells with indicated antibodies of viral restriction factors. ...View More

Nath, P., Chauhan, N. R., Jena, K. K., Datey, A., Kumar, N. D., Mehto, S., ... & Chauhan, S. (2021). Inhibition of IRGM establishes a robust antiviral immune state to restrict pathogenic viruses. EMBO reports, 22(11), e52948.

Rabbit Anti-APOBEC3G Recombinant Antibody (CBYY-C1865) in Immunoblot

2C reduced the expression of A3G. A3G-HA plus VR1012 (negative control) or the indicated EV71 non-structural proteins were co-transfected into H9 cells. The cells were harvested for immunoblotting analysis at 48 h post-transfection. ...View More

Li, Z., Ning, S., Su, X., Liu, X., Wang, H., Liu, Y., ... & Zhang, W. (2018). Enterovirus 71 antagonizes the inhibition of the host intrinsic antiviral factor A3G. Nucleic Acids Research, 46(21), 11514-11527.

Datasheet

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBYY-C1865

Application

Immunogen

Synthetic peptide corresponding to residues surrounding Gly255 of human APOBEC3G protein.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES, pH 7.5, 150 mM NaCl, 100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Apolipoprotein B MRNA Editing Enzyme Catalytic Subunit 3G

Entrez Gene ID

60489

UniProt ID

P17676

Function

DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. Exhibits potent antiviral activity against Vif-deficient HIV-1. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. Exhibits antiviral activity also against simian immunodeficiency viruses (SIVs), hepatitis B virus (HBV), equine infectious anemia virus (EIAV), xenotropic MuLV-related virus (XMRV) and simian foamy virus (SFV). May inhibit the mobility of LTR and non-LTR retrotransposons.

Biological Process

Base conversion or substitution editing Source: HGNC-UCL
Cytidine deamination Source: UniProtKB
Cytidine to uridine editing Source: GO_Central
Defense response to virus Source: UniProtKB
DNA cytosine deamination Source: UniProtKB
DNA demethylation Source: GO_Central
Innate immune response Source: HGNC-UCL
Negative regulation of single stranded viral RNA replication via double stranded DNA intermediate Source: UniProtKB
Negative regulation of transposition Source: UniProtKB
Negative regulation of viral genome replication Source: UniProtKB
Negative regulation of viral process Source: HGNC-UCL
Positive regulation of defense response to virus by host Source: HGNC-UCL
Viral process Source: UniProtKB-KW

Cellular Location

Nucleus; Cytoplasm; P-body. Mainly cytoplasmic. Small amount are found in the nucleus. During HIV-1 infection, virion-encapsidated in absence of HIV-1 Vif.

PTM

Ubiquitinated in the presence of HIV-1 Vif. Association with Vif targets the protein for proteolysis by the ubiquitin-dependent proteasome pathway.
Phosphorylation at Thr-32 reduces its binding to HIV-1 Vif and subsequent ubiquitination and degradation thus promoting its antiviral activity.

Stupfler, B., Verriez, C., Gallois-Montbrun, S., Marquet, R., & Paillart, J. C. (2021). Degradation-Independent Inhibition of APOBEC3G by the HIV-1 Vif Protein. Viruses, 13(4), 617.

Salas Briceno, K., & Ross, S. R. (2021). Repair of APOBEC3G-mutated retroviral DNA in vivo is facilitated by the host enzyme uracil DNA glycosylase 2. Journal of Virology, JVI-01244.

Stupfler, B., Verriez, C., Gallois-Montbrun, S., Marquet, R., & Paillart, J. C. (2021). Degradation-Independent Inhibition of APOBEC3G by the HIV-1 Vif Protein. Viruses 2021, 13, 617.

Botvinnik, A., Shivam, P., Smith, Y., Sharma, G., Olshevsky, U., Moshel, O., ... & Kotler, M. (2021). APOBEC3G rescues cells from the deleterious effects of DNA damage. The FEBS Journal.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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