Mouse Anti-APOF Recombinant Antibody (CBYC-A698) (CBMAB-A3242-YC)

Name: Mouse Anti-APOF Recombinant Antibody (CBYC-A698)
SKU: CBMAB-A3242-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYC-A698

Application

ELISA, IF, WB, IP

Immunogen

Amino acids 44-326 mapping at the C-terminus of apoF of mouse origin.

Host Species

Mouse

Specificity

Mouse

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Apolipoprotein F

Introduction

APOF is one of the minor apolipoproteins found in plasma. This protein forms complexes with lipoproteins and may be involved in transport and/or esterification of cholesterol.

Entrez Gene ID

319

UniProt ID

Q13790

Alternative Names

Apolipoprotein F; Lipid Transfer Inhibitor Protein; Apo-F; LTIP;

Function

Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII.

Biological Process

Cholesterol metabolic process Source: GO_Central
Lipid metabolic process Source: ProtInc
Lipid transport Source: ProtInc

Cellular Location

Secreted

PTM

O-glycosylated with core 1 or possibly core 8 glycans.

Liu, Y., & Morton, R. E. (2020). Apolipoprotein F: A natural inhibitor of cholesteryl ester transfer protein and a key regulator of lipoprotein metabolism. Current Opinion in Lipidology, 31(4), 194-199.

Liu, Y., Izem, L., & Morton, R. E. (2020). Identification of a hormone response element that mediates suppression of APOF by LXR and PPARα agonists. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 1865(3), 158583.

Wang, Y. B., Zhou, B. X., Ling, Y. B., Xiong, Z. Y., Li, R. X., Zhong, Y. S., ... & Deng, M. H. (2019). Decreased expression of ApoF associates with poor prognosis in human hepatocellular carcinoma. Gastroenterology report, 7(5), 354-360.

Morton, R. E., Liu, Y., & Izem, L. (2019). ApoF knockdown increases cholesteryl ester transfer to LDL and impairs cholesterol clearance in fat-fed hamsters. Journal of lipid research, 60(11), 1868-1879.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-APOF Recombinant Antibody (1D5) (CAT#: CBMAB-A3239-YC)

Rabbit Anti-APOF Recombinant Antibody (EG204) (CAT#: CBMAB-EN224-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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