Mouse Anti-APOL3 Recombinant Antibody (8F5) (CBMAB-A0423-LY)

The product is antibody recognizes APOL3. The antibody 8F5 immunoassay techniques such as: WB, ELISA.
See all APOL3 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

8F5

Antibody Isotype

IgG2a, κ

Application

WB, ELISA

Basic Information

Immunogen

APOL3 (NP_663615, 240 a.a. ~ 336 a.a) partial recombinant protein with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

apolipoprotein L, 3

Introduction

This gene is a member of the apolipoprotein L gene family, and it is present in a cluster with other family members on chromosome 6. The encoded protein is found in the cytoplasm, where it may affect the movement of lipids, including cholesterol, and/or allow the binding of lipids to organelles. In addition, expression of this gene is up-regulated by tumor necrosis factor-alpha in endothelial cells lining the normal and atherosclerotic iliac artery and aorta. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Entrez Gene ID

80833

UniProt ID

O95236

Alternative Names

APOLIII; CG12-1

Function

May affect the movement of lipids in the cytoplasm or allow the binding of lipids to organelles.

Biological Process

Inflammatory response Source: ProtInc
Lipoprotein metabolic process Source: InterPro
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB

Cellular Location

Cytoplasm

References

Peng, S. J., Cao, X. K., Dong, D., Liu, M., Hao, D., Shen, X. M., ... & Chen, H. (2020). Integrative analysis of APOL3 gene CNV for adult cattle stature. Animal biotechnology, 31(5), 440-446.

Uzureau, S., Lecordier, L., Uzureau, P., Hennig, D., Graversen, J. H., Homble, F., ... & Pays, E. (2020). APOL1 C-terminal variants may trigger kidney disease through interference with APOL3 control of actomyosin. Cell reports, 30(11), 3821-3836.

Skorecki, K. L., Lee, J. H., Langefeld, C. D., Rosset, S., Tzur, S., Wasser, W. G., ... & Friedman, D. J. (2018). A null variant in the apolipoprotein L3 gene is associated with non-diabetic nephropathy. Nephrology Dialysis Transplantation, 33(2), 323-330.

Khalil, A., Poelvoorde, P., Fayyad-Kazan, M., Rousseau, A., Nuyens, V., Uzureau, S., ... & Vanhamme, L. (2018). Apoliporotein L3 interferes with endothelial tube formation via regulation of ERK1/2, FAK and Akt signaling pathway. Atherosclerosis, 279, 73-87.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
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Second antibody and isotype control

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