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Rabbit Anti-APPL1 Recombinant Antibody (BA0131) (CBMAB-0095CQ)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
BA0131
Antibody Isotype
IgG
Application
IF, IHC, WB, ELISA

Basic Information

Immunogen
A synthetic peptide of human APPL.
Host Species
Rabbit
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IF(ICC)5 µg/ml
IHC5 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% sodium azide
Concentration
Batch dependent
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Introduction
APPL1 contains an N-terminal BAR domain. APPL interacts with the inactive form of AKT2; moreover, APPL binds to the catalytic subunit of PIK3. These data suggested that APPL is an adaptor that may tether inactive AKT2 to the PI3K in the cytoplasm and thereby may expedite recruitment of AKT2 and PI3K to the cell membrane upon mitogenic stimulation. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus.
Entrez Gene ID
Human26060
Mouse72993
Rat290537
UniProt ID
HumanQ9UKG1
MouseQ8K3H0
RatD3ZWA8
Alternative Names
Adaptor Protein, Phosphotyrosine Interaction, PH Domain And Leucine Zipper Containing 1; Adapter Protein Containing PH Domain, PTB Domain And Leucine Zipper Motif 1; Dip13-Alpha; APPL; Adaptor Protein Containing PH Domain, PTB Domain And Leucine Zipper Motif 1; Signaling Adaptor Protein DIP13alpha; AKT2 Interactor; DIP13alpha; KIAA1428; DIP13A
Function
Multifunctional adapter protein that binds to various membrane receptors, nuclear factors and signaling proteins to regulate many processes, such as cell proliferation, immune response, endosomal trafficking and cell metabolism (PubMed:26583432, PubMed:15016378, PubMed:26073777, PubMed:19661063, PubMed:10490823). Regulates signaling pathway leading to cell proliferation through interaction with RAB5A and subunits of the NuRD/MeCP1 complex (PubMed:15016378). Functions as a positive regulator of innate immune response via activation of AKT1 signaling pathway by forming a complex with APPL1 and PIK3R1 (By similarity). Inhibits Fc-gamma receptor-mediated phagocytosis through PI3K/Akt signaling in macrophages (By similarity). Regulates TLR4 signaling in activated macrophages (By similarity). Involved in trafficking of the TGFBR1 from the endosomes to the nucleus via microtubules in a TRAF6-dependent manner (PubMed:26583432). Plays a role in cell metabolism by regulating adiponecting and insulin signaling pathways (PubMed:26073777, PubMed:19661063, PubMed:24879834). Required for fibroblast migration through HGF cell signaling (By similarity). Positive regulator of beta-catenin/TCF-dependent transcription through direct interaction with RUVBL2/reptin resulting in the relief of RUVBL2-mediated repression of beta-catenin/TCF target genes by modulating the interactions within the beta-catenin-reptin-HDAC complex (PubMed:19433865).
Biological Process
Adiponectin-activated signaling pathway Source: UniProtKB
Cell cycle Source: UniProtKB-KW
Cellular response to hepatocyte growth factor stimulus Source: UniProtKB
Extrinsic apoptotic signaling pathway in absence of ligand Source: Reactome
Insulin receptor signaling pathway Source: UniProtKB
Negative regulation of Fc-gamma receptor signaling pathway involved in phagocytosis Source: UniProtKB
Positive regulation of cytokine production involved in inflammatory response Source: UniProtKB
Positive regulation of glucose import Source: UniProtKB
Positive regulation of macropinocytosis Source: UniProtKB
Positive regulation of melanin biosynthetic process Source: UniProtKB
Protein import into nucleus Source: UniProtKB
Regulation of fibroblast migration Source: UniProtKB
Regulation of G1/S transition of mitotic cell cycle Source: UniProtKB
Regulation of glucose import Source: BHF-UCL
Regulation of innate immune response Source: UniProtKB
Regulation of protein localization to plasma membrane Source: BHF-UCL
Regulation of toll-like receptor 4 signaling pathway Source: UniProtKB
Signaling Source: GO_Central
Signal transduction Source: UniProtKB
Transforming growth factor beta receptor signaling pathway Source: UniProtKB
Cellular Location
Cytoplasm; Early endosome membrane; Endosome; Nucleus; Ruffle; Phagosome. Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF.
Involvement in disease
Maturity-onset diabetes of the young 14 (MODY14): A form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
PTM
Phosphorylation at Ser-410 by PKA severely impairs binding to OCRL.
More Infomation

Liu, R., Meng, J., & Lou, D. (2021). Adiponectin inhibits D‑gal‑induced cardiomyocyte senescence via AdipoR1/APPL1. Molecular medicine reports, 24(4), 1-10.

York, H. M., Patil, A., Moorthi, U. K., Kaur, A., Bhowmik, A., Hyde, G. J., ... & Arumugam, S. (2021). Rapid whole cell imaging reveals a calcium-APPL1-dynein nexus that regulates cohort trafficking of stimulated EGF receptors. Communications biology, 4(1), 1-13.

Guo, Q., Chang, B., Yu, Q. L., Xu, S. T., Yi, X. J., & Cao, S. C. (2020). Adiponectin treatment improves insulin resistance in mice by regulating the expression of the mitochondrial-derived peptide MOTS-c and its response to exercise via APPL1–SIRT1–PGC-1α. Diabetologia, 63(12), 2675-2688.

Mohammed, M. A., Mahmoud, M. O., Awaad, A. S., Gamal, G. M., & Abdelfatah, D. (2019). Alpha lipoic acid protects against dexamethasone-induced metabolic abnormalities via APPL1 and PGC-1 α up regulation. Steroids, 144, 1-7.

Sayeed, M., Gautam, S., Verma, D. P., Afshan, T., Kumari, T., Srivastava, A. K., & Ghosh, J. K. (2018). A collagen domain–derived short adiponectin peptide activates APPL1 and AMPK signaling pathways and improves glucose and fatty acid metabolisms. Journal of Biological Chemistry, 293(35), 13509-13523.

Diggins, N. L., & Webb, D. J. (2017). APPL1 is a multifunctional endosomal signaling adaptor protein. Biochemical Society Transactions, 45(3), 771-779.

Jiang, X., Zhou, Y., Wu, K. K., Chen, Z., Xu, A., & Cheng, K. K. (2017). APPL1 prevents pancreatic beta cell death and inflammation by dampening NFκB activation in a mouse model of type 1 diabetes. Diabetologia, 60(3), 464-474.

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For research use only. Not intended for any clinical use.

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