Mouse Anti-AQP4 Recombinant Antibody (CBYC-A724) (CBMAB-A3364-YC)

Name: Mouse Anti-AQP4 Recombinant Antibody (CBYC-A724)
SKU: CBMAB-A3364-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-AQP4 Recombinant Antibody (CBYC-A724) in IF

Representative immunofluorescence labeled images of rabbit (A,C,E) and human (B,D,F) VF epithelium, superficial LP, and intermediate/deep LP labeled for DAPI in blue and AQP1 (A,B), AQP4 (C,D), and AQP7 (E,F) in red. Dotted white boxes represent the magnified portion of image and are displayed in bottom left corner of each image. White arrows indicate other areas of positive labeling. Scale bars: 100 μm. ...View More

Gartling, G. J., Sayce, L., Kimball, E. E., Sueyoshi, S., & Rousseau, B. (2021). A comparison of the localization of integral membrane proteins in human and rabbit vocal folds. The Laryngoscope, 131(4), E1265-E1271.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYC-A724

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 244-323 mapping at the C-terminus of AQP4 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Aquaporin 4

Introduction

AQP4 is a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water home

Entrez Gene ID

Human	361
Mouse	11829
Rat	25293
Rabbit	100008646
Zebrafish	445293

UniProt ID

Human	P55087
Mouse	P55088
Rat	P47863
Rabbit	G1SVZ6
Zebrafish	Q6AZD2

Alternative Names

Aquaporin 4; Mercurial-Insensitive Water Channel; MIWC; WCH4; Aquaporin Type4; Aquaporin-4; AQP-4;

Function

Forms a water-specific channel (PubMed:7559426, PubMed:8601457, PubMed:19383790).
Plays an important role in brain water homeostasis and in glymphatic solute transport. Required for a normal rate of water exchange across the blood brain interface. Required for normal levels of cerebrospinal fluid influx into the brain cortex and parenchyma along paravascular spaces that surround penetrating arteries, and for normal drainage of interstitial fluid along paravenous drainage pathways. Thereby, it is required for normal clearance of solutes from the brain interstitial fluid, including soluble beta-amyloid peptides derived from APP. Plays a redundant role in urinary water homeostasis and urinary concentrating ability (By similarity).

Biological Process

Cellular response to interferon-gamma Source: UniProtKB
Cellular water homeostasis Source: UniProtKB
Cerebrospinal fluid circulation Source: UniProtKB
Multicellular organismal water homeostasis Source: UniProtKB
Protein homotetramerization Source: UniProtKB
Renal water homeostasis Source: Reactome
Water homeostasis Source: UniProtKB
Water transport Source: UniProtKB

Cellular Location

Endosome membrane; Cell membrane; Basolateral cell membrane; Sarcolemma; Cell projection. Activation of the vasopressin receptor AVPR1A triggers AQP4 phosphorylation at Ser-180 and promotes its internalization from the cell membrane. Detected on brain astrocyte processes and astrocyte endfeet close to capillaries.

Topology

Cytoplasmic: 1-36 aa
Helical: 37-57 aa
Extracellular: 58-69 aa
Helical: 70-89 aa
Cytoplasmic: 90-93 aa
Discontinuously helical: 94-101 aa
Cytoplasmic: 102-115 aa
Helical: 116-136 aa
Extracellular: 137-155 aa
Helical: 156-176 aa
Cytoplasmic: 177-184 aa
Helical: 185-205 aa
Extracellular: 206-208 aa
Discontinuously helical: 209-222 aa
Extracellular: 223-231 aa
Helical: 232-252 aa
Cytoplasmic: 253-323 aa

PTM

Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamate increases conductance by 40% (By similarity).
Isoform 2: Palmitoylated on its N-terminal region. Isoform 1: Not palmitoylated.

Durozard, P., Rico, A., Boutiere, C., Maarouf, A., Lacroix, R., Cointe, S., ... & Audoin, B. (2020). Comparison of the response to rituximab between myelin oligodendrocyte glycoprotein and aquaporin‐4 antibody diseases. Annals of neurology, 87(2), 256-266.

Sotirchos, E. S., Filippatou, A., Fitzgerald, K. C., Salama, S., Pardo, S., Wang, J., ... & Saidha, S. (2020). Aquaporin-4 IgG seropositivity is associated with worse visual outcomes after optic neuritis than MOG-IgG seropositivity and multiple sclerosis, independent of macular ganglion cell layer thinning. Multiple Sclerosis Journal, 26(11), 1360-1371.

Duan, T., Tradtrantip, L., Phuan, P. W., Bennett, J. L., & Verkman, A. S. (2020). Affinity-matured ‘aquaporumab’anti-aquaporin-4 antibody for therapy of seropositive neuromyelitis optica spectrum disorders. Neuropharmacology, 162, 107827.

Tamtaji, O. R., Behnam, M., Pourattar, M. A., Jafarpour, H., & Asemi, Z. (2019). Aquaporin 4: A key player in Parkinson's disease. Journal of cellular physiology, 234(12), 21471-21478.

Nakano, T., Nishigami, C., Irie, K., Shigemori, Y., Sano, K., Yamashita, Y., ... & Mishima, K. (2018). Goreisan prevents brain edema after cerebral ischemic stroke by inhibiting aquaporin 4 upregulation in mice. Journal of Stroke and Cerebrovascular Diseases, 27(3), 758-763.

Wilson, R., Makuch, M., Kienzler, A. K., Varley, J., Taylor, J., Woodhall, M., ... & Irani, S. R. (2018). Condition-dependent generation of aquaporin-4 antibodies from circulating B cells in neuromyelitis optica. Brain, 141(4), 1063-1074.

Duignan, S., Wright, S., Rossor, T., Cazabon, J., Gilmour, K., Ciccarelli, O., ... & Hacohen, Y. (2018). Myelin oligodendrocyte glycoprotein and aquaporin‐4 antibodies are highly specific in children with acquired demyelinating syndromes. Developmental Medicine & Child Neurology, 60(9), 958-962.

Salman, M. M., Kitchen, P., Woodroofe, M. N., Brown, J. E., Bill, R. M., Conner, A. C., & Conner, M. T. (2017). Hypothermia increases aquaporin 4 (AQP 4) plasma membrane abundance in human primary cortical astrocytes via a calcium/transient receptor potential vanilloid 4 (TRPV 4)‐and calmodulin‐mediated mechanism. European Journal of Neuroscience, 46(9), 2542-2547.

Eidsvaag, V. A., Enger, R., Hansson, H. A., Eide, P. K., & Nagelhus, E. A. (2017). H uman and mouse cortical astrocytes differ in aquaporin‐4 polarization toward microvessels. Glia, 65(6), 964-973.

Verkman, A. S., Smith, A. J., Phuan, P. W., Tradtrantip, L., & Anderson, M. O. (2017). The aquaporin-4 water channel as a potential drug target in neurological disorders. Expert opinion on therapeutic targets, 21(12), 1161-1170.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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