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Mouse Anti-ARF3 Recombinant Antibody (41/ARF3) (CBMAB-A3427-YC)

Provided herein is a Mouse monoclonal antibody against Human ADP Ribosylation Factor 3. The antibody can be used for immunoassay techniques, such as WB.
See all ARF3 antibodies
Published Data
Mouse Anti-ARF3 Recombinant Antibody (41/ARF3) CBMAB-A3427-YC in WB
Images were processed and data analyzed using KNIME software. Changes in Golgi area were calculated relative to control (DMSO) by dividing area mean values of AMF26-treated cells by area mean values of vehicle-treated cells derived from the Golgicovering mask (for more details, see Materials and Methods) based on three wells counting on average 1000 cells per cell line and condition. Shown is a representative graph of two independent experiments. Error bars represent S.D. ...View More
Ignashkova, T. I., Gendarme, M., Peschk, K., Eggenweiler, H. M., Lindemann, R. K., & Reiling, J. H. (2017). Cell survival and protein secretion associated with Golgi integrity in response to Golgi stress-inducing agents. Traffic, 18(8), 530-544.

Summary

Host Animal
Mouse
Specificity
Human, Rat, Dog, Mouse
Clone
41/ARF3
Antibody Isotype
IgG1, κ
Application
WB

Basic Information

Immunogen
Amino acids 1-181 representing full length ARF3 of human origin.
Specificity
Human, Rat, Dog, Mouse
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ADP Ribosylation Factor 3
Introduction
ADP-ribosylation factor 3 (ARF3) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as act
Entrez Gene ID
Human377
Mouse11842
Rat140940
Chicken107049507
Dog486562
Frog100145191
UniProt ID
HumanP61204
MouseP61205
RatP61206
ChickenA0A3Q2U6I8
DogE2RSF6
FrogB0JZE7
Alternative Names
ADP Ribosylation Factor 3; Small GTP Binding Protein;
Function
GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.
Biological Process
Intracellular protein transport Source: GO_Central
Phosphatidylinositol biosynthetic process Source: Reactome
Retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: WormBase
Vesicle-mediated transport Source: GO_Central
Cellular Location
Golgi apparatus; Perinuclear region
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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