Sign in or Register   Sign in or Register
  |  

Mouse Anti-ARF5 Recombinant Antibody (1B4) (CBMAB-A3430-YC)

Provided herein is a Mouse monoclonal antibody against Human ADP Ribosylation Factor 5. The antibody can be used for immunoassay techniques, such as ELISA, IF, WB.
See all ARF5 antibodies
Published Data
Mouse Anti-ARF5 Recombinant Antibody (1B4) CBMAB-A3430-YC in WB
Images were processed and data analyzed using KNIME software. Changes in Golgi area were calculated relative to control (DMSO) by dividing area mean values of AMF26-treated cells by area mean values of vehicle-treated cells derived from the Golgicovering mask (for more details, see Materials and Methods) based on three wells counting on average 1000 cells per cell line and condition. Shown is a representative graph of two independent experiments. Error bars represent S.D. ...View More
Ignashkova, T. I., Gendarme, M., Peschk, K., Eggenweiler, H. M., Lindemann, R. K., & Reiling, J. H. (2017). Cell survival and protein secretion associated with Golgi integrity in response to Golgi stress-inducing agents. Traffic, 18(8), 530-544.
Mouse Anti-ARF5 Recombinant Antibody (1B4) CBMAB-A3430-YC in IF
Localization of Arf isoforms during cytokinesis. HeLa cells were fixed and permeabilized as described in Materials and Methods doubly stained with anti-β-tubulin antibody (middle panels) and antibody against Arf1, Arf3, Arf4, Arf5 or Arf6 (left panels). Merged images are shown in right panels. Bar = 10 µm. ...View More
Hanai, A., Ohgi, M., Yagi, C., Ueda, T., Shin, H. W., & Nakayama, K. (2016). Class I Arfs (Arf1 and Arf3) and Arf6 are localized to the Flemming body and play important roles in cytokinesis. The journal of biochemistry, 159(2), 201-208.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
1B4
Antibody Isotype
IgG1, κ
Application
ELISA, IF, WB

Basic Information

Immunogen
Partial recombinant corresponding to aa 81-180 from human ARF5 (AAH03043) with GST tag.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IF(ICC)15 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ADP-ribosylation factor 5
Introduction
ARF5 belongs to the human ADP-ribosylation factor (ARF) gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activator
Entrez Gene ID
Human381
Mouse11844
Rat79117
UniProt ID
HumanP84085
MouseP84084
RatP84083
Alternative Names
ADP Ribosylation Factor 5;
Function
GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.
(Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.
Biological Process
Intracellular protein transport Source: GO_Central
Retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: WormBase
Vesicle-mediated transport Source: GO_Central
Cellular Location
Golgi apparatus; Trans-Golgi network membrane; perinuclear region; Membrane
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-ARF5 Recombinant Antibody (1B4)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare