Mouse Anti-ARF5 Recombinant Antibody (1B4) (CBMAB-A3430-YC)

Name: Mouse Anti-ARF5 Recombinant Antibody (1B4)
SKU: CBMAB-A3430-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ARF5 Recombinant Antibody (1B4) CBMAB-A3430-YC in WB

Images were processed and data analyzed using KNIME software. Changes in Golgi area were calculated relative to control (DMSO) by dividing area mean values of AMF26-treated cells by area mean values of vehicle-treated cells derived from the Golgicovering mask (for more details, see Materials and Methods) based on three wells counting on average 1000 cells per cell line and condition. Shown is a representative graph of two independent experiments. Error bars represent S.D. ...View More

Ignashkova, T. I., Gendarme, M., Peschk, K., Eggenweiler, H. M., Lindemann, R. K., & Reiling, J. H. (2017). Cell survival and protein secretion associated with Golgi integrity in response to Golgi stress-inducing agents. Traffic, 18(8), 530-544.

Mouse Anti-ARF5 Recombinant Antibody (1B4) CBMAB-A3430-YC in IF

Localization of Arf isoforms during cytokinesis. HeLa cells were fixed and permeabilized as described in Materials and Methods doubly stained with anti-β-tubulin antibody (middle panels) and antibody against Arf1, Arf3, Arf4, Arf5 or Arf6 (left panels). Merged images are shown in right panels. Bar = 10 µm. ...View More

Hanai, A., Ohgi, M., Yagi, C., Ueda, T., Shin, H. W., & Nakayama, K. (2016). Class I Arfs (Arf1 and Arf3) and Arf6 are localized to the Flemming body and play important roles in cytokinesis. The journal of biochemistry, 159(2), 201-208.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1B4

Application

ELISA, IF, WB

Immunogen

Partial recombinant corresponding to aa 81-180 from human ARF5 (AAH03043) with GST tag.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF(ICC)	15 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ADP-ribosylation factor 5

Introduction

ARF5 belongs to the human ADP-ribosylation factor (ARF) gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activator

Entrez Gene ID

Human	381
Mouse	11844
Rat	79117

UniProt ID

Human	P84085
Mouse	P84084
Rat	P84083

Alternative Names

ADP Ribosylation Factor 5;

Function

GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.
(Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.

Biological Process

Intracellular protein transport Source: GO_Central
Retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: WormBase
Vesicle-mediated transport Source: GO_Central

Cellular Location

Golgi apparatus; Trans-Golgi network membrane; perinuclear region; Membrane

Zhou, C. X., Wang, Y., Shi, L. Y., Wang, Z. B., Ma, Y., Li, C. R., ... & Xia, Z. R. (2021). GTPases Arf5 and Arl2 function partially distinctly during oocyte meiosis. Journal of Cellular Biochemistry, 122(2), 198-208.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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