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Mouse Anti-ARF6 Recombinant Antibody (EG221) (CBMAB-EN248-LY)

The product is antibody recognizes ARF6 . The antibody EG221 immunoassay techniques such as: WB: 1:500~1:1000 ELISA: 1:10000.
See all ARF6 antibodies
Published Data
Mouse Anti-ARF6 Recombinant Antibody (EG221) CBMAB-EN248-LY in WB
Immunodetection of extracellular vesicle (EV) markers in whole-cell protein extracts (lines C) and exosome samples (lines E). Prior to immunodetection, 50 µg of protein per line was separated by 10% SDS-PAGE and transferred into the PVDF membrane. Immunodetection was performed with the use of the following primary antibodies: anti CD9, anti-CD63, anti-Hsp70, and anti-Arf6, and anti-mouse IgG-HRP as a secondary antibody. ...View More
Surman, M., Jankowska, U., Wilczak, M., & Przybyło, M. (2023). Similarities and differences in the protein composition of cutaneous melanoma cells and their exosomes identified by mass spectrometry. Cancers, 15(4), 1097.
Mouse Anti-ARF6 Recombinant Antibody (EG221) CBMAB-EN248-LY in IF
ARF6, ARNO and FE65 colocalize in growth cone. Rat embryonic cortical neurons were immunostained at DIV3 for the detection of endogenous ARF6, ARNO and FE65 using mouse anti-ARF6 3A-1, rat anti-ARNO, rabbit anti-FE65, respectively. Zoomed area of boxes with growth cones are shown. Scale bar is 10 µm. A representative neuron is shown. ...View More
Zhai, Y., Chan, W. W. R., Li, W., & Lau, K. F. (2022). ARNO is recruited by the neuronal adaptor FE65 to potentiate ARF6-mediated neurite outgrowth. Open Biology, 12(9), 220071.
Mouse Anti-ARF6 Recombinant Antibody (EG221) CBMAB-EN248-LY in IF
The ERC localization of ARNO is enhanced by FE65. HEK293 cells were transfected with either ARNO + ARF6, ARNO + ARF6 + FE65 or ARNO + ARF6 + FE65R605A. ...View More
Zhai, Y., Chan, W. W. R., Li, W., & Lau, K. F. (2022). ARNO is recruited by the neuronal adaptor FE65 to potentiate ARF6-mediated neurite outgrowth. Open Biology, 12(9), 220071.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
EG221
Antibody Isotype
IgG2b, κ
Application
ELISA, IF, IP, WB, IHC-P

Basic Information

Immunogen
Amino acids 1-174 representing full length of ARF6 of human origin.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
ADP Ribosylation Factor 6
Introduction
This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human382
Mouse11845
Rat79121
UniProt ID
HumanP62330
MouseP62331
RatP62332
Alternative Names
ADP Ribosylation Factor 6;
Function
GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling (PubMed:11266366, PubMed:21170023, PubMed:16737952, PubMed:7589240, PubMed:18400762).
Required for normal completion of mitotic cytokinesis (By similarity).
Plays a role in the reorganization of the actin cytoskeleton and the formation of stress fibers (By similarity).
Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension (PubMed:14978216).
Plays an important role in membrane trafficking, during junctional remodeling and epithelial polarization. Regulates surface levels of adherens junction proteins such as CDH1 (By similarity).
Required for NTRK1 sorting to the recycling pathway from early endosomes (By similarity).
(Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.
Biological Process
Cell adhesion Source: UniProtKB
Cell cycle Source: UniProtKB-KW
Cell differentiation Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Cellular response to nerve growth factor stimulus Source: Ensembl
Cortical actin cytoskeleton organization Source: UniProtKB
Endocytic recycling Source: UniProtKB
Establishment of epithelial cell polarity Source: Ensembl
Hepatocyte apoptotic process Source: Ensembl
Intracellular protein transport Source: GO_Central
Liver development Source: Ensembl
Maintenance of postsynaptic density structure Source: Ensembl
Myeloid cell apoptotic process Source: Ensembl
Negative regulation of dendrite development Source: Ensembl
Negative regulation of protein localization to cell surface Source: Ensembl
Negative regulation of receptor-mediated endocytosis Source: UniProtKB
Nervous system development Source: UniProtKB-KW
Positive regulation of actin filament polymerization Source: UniProtKB
Positive regulation of focal adhesion disassembly Source: ARUK-UCL
Positive regulation of keratinocyte migration Source: ARUK-UCL
Positive regulation of protein localization to plasma membrane Source: BHF-UCL
Positive regulation of protein secretion Source: UniProtKB
Protein localization to cell surface Source: BHF-UCL
Protein localization to endosome Source: UniProtKB
Regulation of dendritic spine development Source: UniProtKB
Regulation of filopodium assembly Source: UniProtKB
Regulation of presynapse assembly Source: Ensembl
Regulation of Rac protein signal transduction Source: UniProtKB
Ruffle assembly Source: UniProtKB
Synaptic vesicle endocytosis Source: Ensembl
Vesicle-mediated transport Source: GO_Central
Cellular Location
Endosome membrane; Recycling endosome membrane; Early endosome membrane; Trans-Golgi network membrane; Cell membrane; Filopodium membrane; Cytosol; Ruffle; Cleavage furrow; Midbody ring. Distributed uniformly on the plasma membrane, as well as throughout the cytoplasm during metaphase. Subsequently concentrated at patches in the equatorial region at the onset of cytokinesis, and becomes distributed in the equatorial region concurrent with cleavage furrow ingression. In late stages of cytokinesis, concentrates at the midbody ring/Flemming body (PubMed:23603394). Recruitment to the midbody ring requires both activation by PSD/EFA6A and interaction with KIF23/MKLP1 (By similarity). After abscission of the intercellular bridge, incorporated into one of the daughter cells as a midbody remnant and localizes to punctate structures beneath the plasma membrane (PubMed:23603394). Recruited to the cell membrane in association with CYTH2 and ARL4C. Colocalizes with DAB2IP at the plasma membrane and endocytic vesicles (By similarity). Myristoylation is required for proper localization to membranes (PubMed:7589240).

Wang, R., Schneider, S., Keppler, O. T., Li, B., Rutz, B., Ciotkowska, A., ... & Hennenberg, M. (2021). ADP ribosylation factor 6 promotes contraction and proliferation, suppresses apoptosis and is specifically inhibited by NAV2729 in prostate stromal cells. Molecular Pharmacology.

Ito, A., Fukaya, M., Sugawara, T., Hara, Y., Okamoto, H., Yamauchi, J., & Sakagami, H. (2021). Cytohesin-2 mediates group I metabotropic glutamate receptor-dependent mechanical allodynia through the activation of ADP ribosylation factor 6 in the spinal cord. Neurobiology of Disease, 105466.

Yu*, Q., Gratzke, C., Kuppermann, P., Herlemann, A., Tamalunas, A., Wang, Y., ... & Hennenberg, M. (2019). MP06-10 INHIBITION OF PROSTATE SMOOTH MUSCLE CONTRACTION BY NAV2729 SUGGESTS PROMOTION OF PROSTATE SMOOTH MUSCLE CONTRACTION BY ADP RIBOSYLATION FACTOR 6. The Journal of Urology, 201(Supplement 4), e51-e51.

Rahmati, M., Shariatzadeh, M., Kazemi, A., & Taherabadi, S. J. (2019). High-intensity interval training increasing ADP-ribosylation factor 6 and Cytochrome C in visceral adipose tissue of male Wistar rats. Obesity Medicine, 14, 100089.

Taherabadi, S. J., Rahmati, M., & Mirnasuri, R. (2018). Effect of endurance training on cerebellar gene expression of the ADP-ribosylation factor 6 in rats with diabetic peripheral neuropathy. Zahedan Journal of Research in Medical Sciences, 20(12).

Uehara, S., Saito, K., Asami, H., & Ohta, Y. (2017). Role of ARHGAP24 in ADP ribosylation factor 6 (ARF6)-dependent pseudopod formation in human breast carcinoma cells. Anticancer research, 37(9), 4837-4844.

Li, R., Peng, C., Zhang, X., Wu, Y., Pan, S., & Xiao, Y. (2017). Roles of Arf6 in cancer cell invasion, metastasis and proliferation. Life sciences, 182, 80-84.

Katsumata, O., Mori, M., Sawane, Y., Niimura, T., Ito, A., Okamoto, H., ... & Sakagami, H. (2017). Cellular and subcellular localization of ADP-ribosylation factor 6 in mouse peripheral tissues. Histochemistry and cell biology, 148(6), 577-596.

Alhadidy, M. M., Reiser, M., Szekeres, C., Cha, B., & Noujaim, S. F. (2017). ADP Ribosylation Factor 6 (Arf6) Modulates the Cell Surface Expression of the Cardiac Acetylcholine‐Activated Inward Rectifier Potassium Current (IKACh) Proteins. The FASEB Journal, 31, 991-8.

Chakraborti, S., Sarkar, J., Chowdhury, A., & Chakraborti, T. (2017). Role of ADP ribosylation factor6− Cytohesin1− PhospholipaseD signaling axis in U46619 induced activation of NADPH oxidase in pulmonary artery smooth muscle cell membrane. Archives of biochemistry and biophysics, 633, 1-14.

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For research use only. Not intended for any clinical use.

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