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Mouse Anti-ARFGAP2 Recombinant Antibody (B-6) (CBMAB-A3451-YC)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
B-6
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 373-414 within an internal region of ARFGAP2 of human origin.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ADP Ribosylation Factor GTPase Activating Protein 2
Introduction
GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Implicated in coatomer-mediated protein transport between the Golgi complex and the endoplasmic reticulum. Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-d
Entrez Gene ID
Human84364
Mouse77038
Rat362162
UniProt ID
HumanQ8N6H7
MouseQ99K28
RatQ3MID3
Alternative Names
ADP Ribosylation Factor GTPase Activating Protein 2; Zinc Finger Protein 289, ID1 Regulated; GTPase-Activating Protein ZNF289; ZNF289; ADP-Ribosylation Factor GTPase-Activating Protein 2; Zinc Finger Protein 289;
Function
GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Implicated in coatomer-mediated protein transport between the Golgi complex and the endoplasmic reticulum. Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.
Biological Process
COPI coating of Golgi vesicle Source: GO_Central
Endoplasmic reticulum to Golgi vesicle-mediated transport Source: Reactome
Protein transport Source: UniProtKB-KW
Retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: Reactome
Cellular Location
Cytoplasm; Golgi apparatus membrane. Also found on peripheral punctate structures likely to be endoplasmic reticulum-Golgi intermediate compartment.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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