Mouse Anti-ARFGEF1 Recombinant Antibody (3E11) (CBMAB-A3453-YC)

Provided herein is a Mouse monoclonal antibody against Human ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 1. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all ARFGEF1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

3E11

Antibody Isotype

IgG2a, κ

Application

ELISA, WB

Basic Information

Immunogen

ARFGEF1 (NP_006412. 2, 311-411 aa) partial recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 311-411

Target

Full Name

ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited)

Introduction

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP. It contains a Sec7 domain, which may b

Entrez Gene ID

10565

UniProt ID

Q9Y6D6

Alternative Names

ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 1; ADP-Ribosylation Factor Guanine Nucleotide-Exchange Factor 1 (Brefeldin A-Inhibited); Brefeldin A-Inhibited Guanine Nucleotide-Exchange Protein 1; P200 ARF Guanine Nucleotide Exchange Factor; A

Function

Promotes guanine-nucleotide exchange on ARF1 and ARF3. Promotes the activation of ARF1/ARF3 through replacement of GDP with GTP. Involved in vesicular trafficking. Required for the maintenance of Golgi structure; the function may be independent of its GEF activity. Required for the maturaion of integrin beta-1 in the Golgi. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways. Inhibits GAP activity of MYO9B probably through competetive RhoA binding. The function in the nucleus remains to be determined.

Biological Process

Endomembrane system organization Source: UniProtKB
Exocytosis Source: ProtInc
Golgi organization Source: UniProtKB
Negative regulation of actin filament polymerization Source: UniProtKB
Negative regulation of GTPase activity Source: UniProtKB
Neuron projection development Source: Ensembl
Positive regulation of GTPase activity Source: GOC
Positive regulation of protein glycosylation in Golgi Source: UniProtKB
Positive regulation of protein kinase B signaling Source: Ensembl
Positive regulation of wound healing Source: UniProtKB
Protein transport Source: UniProtKB-KW
Regulation of ARF protein signal transduction Source: InterPro
Regulation of establishment of cell polarity Source: UniProtKB

Cellular Location

Cytoplasm; Golgi apparatus; Trans-Golgi network membrane; Nucleus; Nucleolus; Nucleus matrix; Perinuclear region. Translocates from cytoplasm to membranes and nucleus upon cAMP treatment.

PTM

Phosphorylated. In vitro phosphorylated by PKA reducing its GEF activity and dephosphorylated by phosphatase PP1.

References

Thomas, Q., Gautier, T., Marafi, D., Besnard, T., Willems, M., Moutton, S., ... & Vitobello, A. (2021). Haploinsufficiency of ARFGEF1 is associated with developmental delay, intellectual disability, and epilepsy with variable expressivity. Genetics in Medicine, 1-11.

Teoh, J., Subramanian, N., Pero, M. E., Bartolini, F., Amador, A., Kanber, A., ... & Frankel, W. N. (2020). Arfgef1 haploinsufficiency in mice alters neuronal endosome composition and decreases membrane surface postsynaptic GABAA receptors. Neurobiology of disease, 134, 104632.

Han, J., Zhang, M., Nie, C., Jia, J., Wang, F., Yu, J., ... & Zhang, J. (2019). miR-215 suppresses papillary thyroid cancer proliferation, migration, and invasion through the AKT/GSK-3β/Snail signaling by targeting ARFGEF1. Cell death & disease, 10(3), 1-12.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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