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Rabbit Anti-ARFGEF2 Recombinant Antibody (CBYC-A745) (CBMAB-A3454-YC)

Provided herein is a Rabbit monoclonal antibody against Human ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 2. The antibody can be used for immunoassay techniques, such as WB.
See all ARFGEF2 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
CBYC-A745
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
A synthetic peptide of human ARFGEF2.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
50mM Tris-Glycine, pH 7.4, 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% sodium azide
Concentration
0.3 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 2
Introduction
ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. It
Entrez Gene ID
Human10564
Mouse99371
Rat296380
UniProt ID
HumanQ9Y6D5
MouseA2A5R2
RatQ7TSU1
Alternative Names
ADP Ribosylation Factor Guanine Nucleotide Exchange Factor 2; ADP-Ribosylation Factor Guanine Nucleotide-Exchange Factor 2 (Brefeldin A-Inhibited); Brefeldin A-Inhibited Guanine Nucleotide-Exchange Protein 2; Brefeldin A-Inhibited GEP 2; BIG2; ADP-Ribosyl
Function
Promotes guanine-nucleotide exchange on ARF1 and ARF3 and to a lower extent on ARF5 and ARF6. Promotes the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. Involved in the regulation of Golgi vesicular transport. Required for the integrity of the endosomal compartment. Involved in trafficking from the trans-Golgi network (TGN) to endosomes and is required for membrane association of the AP-1 complex and GGA1. Seems to be involved in recycling of the transferrin receptor from recycling endosomes to the plasma membrane. Probably is involved in the exit of GABA(A) receptors from the endoplasmic reticulum. Involved in constitutive release of tumor necrosis factor receptor 1 via exosome-like vesicles; the function seems to involve PKA and specifically PRKAR2B. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways.
Biological Process
Endomembrane system organization Source: UniProtKB
Endosome organization Source: UniProtKB
Exocytosis Source: ProtInc
Golgi to plasma membrane transport Source: UniProtKB
Intracellular signal transduction Source: MGI
Positive regulation of tumor necrosis factor production Source: UniProtKB
Protein transport Source: UniProtKB-KW
Receptor recycling Source: UniProtKB
Regulation of ARF protein signal transduction Source: InterPro
Cellular Location
Cytoplasm; Endosome; Golgi apparatus; Trans-Golgi network; Centrosome; Cytoskeleton; Membrane; Perinuclear region; Dendrite; Cytoplasmic vesicle; Synapse. Translocates from cytoplasm to membranes upon cAMP treatment. Localized in recycling endosomes.
Involvement in disease
Periventricular nodular heterotopia 2 (PVNH2): A developmental disorder characterized by the presence of periventricular nodules of cerebral gray matter, resulting from a failure of neurons to migrate normally from the lateral ventricular proliferative zone, where they are formed, to the cerebral cortex. PVNH2 is an autosomal recessive form characterized by microcephaly (small brain), severe developmental delay and recurrent infections. No anomalies extrinsic to the central nervous system, such as dysmorphic features or grossly abnormal endocrine or other conditions, are associated with PVNH2.
PTM
In vitro phosphorylated by PKA reducing its GEF activity and dephosphorylated by phosphatase PP1.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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