Mouse Anti-ARHGAP1 Recombinant Antibody (A-9) (CBMAB-1139-CN)

This product is a mouse antibody that recognizes ARHGAP1 of human. The antibody A-9 can be used for immunoassay techniques such as: WB, IP, IF, ELISA.
See all ARHGAP1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

A-9

Antibody Isotype

IgG1, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 1-76 mapping at the N-terminus of ARHGAP1 of human origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Rho GTPase Activating Protein 1

Introduction

This gene encodes a member of a large family of proteins that activate Rho-type guanosine triphosphate (GTP) metabolizing enzymes. The encoded protein contains a SRC homology 3 domain and interacts with Bcl-2-associated protein family members. It is a GTPase activator for the Rho, Rac and Cdc42 proteins, converting them to the putatively inactive GDP-bound state. Cdc42 seems to be the preferred substrate.

Entrez Gene ID

Human	392
Mouse	228359
Rat	311193

UniProt ID

Human	Q07960
Mouse	Q5FWK3
Rat	D4A6C5

Alternative Names

RHOGAP; RHOGAP1; CDC42GAP; p50rhoGAP

Function

GTPase activator for the Rho, Rac and Cdc42 proteins, converting them to the putatively inactive GDP-bound state. Cdc42 seems to be the preferred substrate.

Biological Process

Endosomal transport Source: CAFA
Negative regulation of endocytic recycling Source: CAFA
Regulation of small GTPase mediated signal transduction Source: Reactome
Rho protein signal transduction Source: ProtInc
Small GTPase mediated signal transduction Source: MGI
Transferrin transport Source: CAFA

Cellular Location

Cytoplasm

References

Qi, L., Sun, B., Yang, B., & Lu, S. (2021). circRNA RPPH1 Facilitates the Aggravation of Breast Cancer Development by Regulating miR-542-3p/ARHGAP1 Pathway. Cancer Biotherapy & Radiopharmaceuticals.

Yang, C., Zhang, G., Zhang, Y., Zhang, S., Li, J., & Liu, Y. (2021). Exosome miR‐134‐5p restrains breast cancer progression via regulating PI3K/AKT pathway by targeting ARHGAP1. Journal of Obstetrics and Gynaecology Research.

Liang, M., Yin, X., Zhang, S., Ai, H., Luo, F., Xu, J., ... & Ma, Q. (2021). Osteoclast-derived small extracellular vesicles induce osteogenic differentiation via inhibiting ARHGAP1. Molecular Therapy-Nucleic Acids, 23, 1191-1203.

Zhang, X., Guan, J., Guo, M., Dai, H., Cai, S., Zhou, C., ... & Qin, Q. (2019). Rho GTPase‑activating protein 1 promotes apoptosis of myocardial cells in an ischemic cardiomyopathy model. Kardiologia Polska (Polish Heart Journal), 77(12), 1163-1169.

Satterfield, L., Shuck, R., Kurenbekova, L., Allen‐Rhoades, W., Edwards, D., Huang, S., ... & Yustein, J. T. (2017). miR‐130b directly targets Arhgap1 to drive activation of a metastatic CDC42‐PAK1‐AP1 positive feedback loop in Ewing sarcoma. International journal of cancer, 141(10), 2062-2075.

Li, J. P., Liu, Y., & Yin, Y. H. (2017). ARHGAP1 overexpression inhibits proliferation, migration and invasion of C-33A and SiHa cell lines. OncoTargets and therapy, 10, 691.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-ARHGAP1 Recombinant Antibody (0.T.135)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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