Mouse Anti-ARHGAP36 Recombinant Antibody (12D10.1) (CBMAB-A3496-YC)

Name: Mouse Anti-ARHGAP36 Recombinant Antibody (12D10.1)
SKU: CBMAB-A3496-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

12D10.1

Application

IHC, WB

Immunogen

Recombinant protein fragment corresponding to human ARHGAP36 at C-terminal, fused to GST-Tag

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl

Preservative

0.05% sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Rho GTPase Activating Protein 36

Introduction

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.

Entrez Gene ID

158763

UniProt ID

Q6ZRI8

Alternative Names

Rho GTPase Activating Protein 36;

Function

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.

Biological Process

Positive regulation of GTPase activity Source: GOC
Regulation of small GTPase mediated signal transduction Source: Reactome
Signal transduction Source: InterPro

Cellular Location

Cytosol

Nano, P. R., Johnson, T. K., Kudo, T., Mooney, N. A., Ni, J., Demeter, J., ... & Chen, J. K. (2021). Structure-activity mapping of ARHGAP36 reveals regulatory roles for its GAP homology and C-terminal domains. PloS one, 16(5), e0251684.

Nano, P. R., Kudo, T., Mooney, N. A., Ni, J., Demeter, J., Jackson, P. K., & Chen, J. K. (2020). Multiple domains in ARHGAP36 regulate PKA degradation and Gli activation. bioRxiv.

Nam, H., Jeon, S., An, H., Yoo, J., Lee, H. J., Lee, S. K., & Lee, S. (2019). Critical roles of ARHGAP36 as a signal transduction mediator of Shh pathway in lateral motor columnar specification. Elife, 8, e46683.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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